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Genotype analysis of genes involved in increasing grain number per panicle in rice germplasm
J Plant Biotechnol 2017;44:356-363
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Dongjin Shin1・Tae-Heon Kim1・Ji-Yoon Lee1・Jun-Hyun Cho1・Jong-Hee Lee2・You-Chun Song1・Dong-soo Park1・Myeong-Kyu Oh1

1Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang, 50424, Korea
2Research Policy Bureau, RDA, Jeonju, 54875, Korea
Correspondence to: D. J Shin (⊠)
Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang, 50424, Korea
Dongjin Shin and Tae-Heon Kim equally contributed to this work.
Received November 7, 2017; Revised November 13, 2017; Accepted November 13, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
ARice is an important staple food in the world and rice yield is one of the main traits for rice breeding. Several genes involved in increasing the yield have been identified through map-based gene cloning within natural variations in rice. These identified genes are good targets for introducing a genetic trait in molecular breeding. Here, we chose five genes reported to be involved in increasing grain number per panicle in rice; Gn1a, dep1, Apo1, Ghd7, and Nal1. We developed In/Del markers for Gn1a, and dep1, Apo1, and applied the reported SNP markers for Ghd7 and Nal1. We were easily able to examine the genotype of each gene on agarose gel. We tested the genotypes on 479 rice resources that we held with evaluated molecular markers. According to the genotype of each gene, rice resources were divided into 13 haplotypes, and most of the Indica and Japonica varieties were included in haplotypes 1 and 13, respectively. When we examined the effect of each gene on grain number per panicle and panicle number per plant, panicle number per plant in the yield negative allele group for each gene was reduced by approximately 0.3 to 0.8 compared to that in the yield positive allele group. However, the number of yield positive alleles for each gene was higher by about 21 to 27 grains per panicle than that of yield negative alleles. Although most of the varieties were grouped in haplotypes 1 and 13, we believe that this genotype information with evaluated molecular markers will be useful in rice breeding for increasing the yield with grain number per panicle.
Keywords : Rice, Grain number per panicle, Yield, Molecular marker

December 2017, 44 (4)
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