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Evaluation of resistance to Pierce’s disease among grapevine cultivars by using the culture filtrates produced from Xylella fastidiosa
J Plant Biotechnol 2017;44:394-400
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Myung Soo Park1・Jiang Lu2・Hae Keun Yun3

1School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Korea
2School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China, and Viticulture Center, Florida A&M University, FL32308, USA
3Department of Horticulture and Life Science, Yeungnam University, Kyeongsan 38541, Korea
Correspondence to: H. K. Yun (⊠)
Department of Horticulture and Life Science, Yeungnam University, Kyeongsan 38541, Korea)
e-mail: haekeun@ynu.ac.kr
Contributed equally
Received November 3, 2017; Revised November 29, 2017; Accepted November 29, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study investigated whether culture filtrates produced by Xylella fastidiosa can be used to determine the varietal susceptibility to Pierce's disease in grapevines (Vitis spp.) as a substitute for pathogen inoculation or field screening. A bioassay of grape leaves with culture filtrates from the pathogen showed that their phytotoxicities were active and host-selective. Ethyl acetate extracts from them also showed toxicities and host selectivity in both bunches of grapes and muscadine grapes. The sensitive range of plants to the culture filtrates and their ethyl acetate extracts was consistent with the host range of the Pierce’s disease pathogen. Susceptible cultivars are sensitive to even highly diluted culture filtrates, while resistant cultivars were not affected even at their original culture filtrates. Susceptible cultivars were more sensitive to the undiluted culture filtrate than were highly diluted culture filtrates, and the younger leaves were the most sensitive to the culture filtrates in grapes. Although some European grape cultivars showed moderately susceptibility in this study, the determination of varietal resistance to Pierce’s disease by the treatment of culture filtrates of pathogens could provide valuable information for the preliminary selection of genetic resources and seedlings from hybridization in a disease resistant grape breeding program.
Keywords : Disease resistance, Genetic resources, Phytotoxicity, Screening, Vitis


December 2017, 44 (4)
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