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Improvement of ex vitro acclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium
J Plant Biotechnol 2017;44:431-437
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Yoon Sun Huh・Joung Kwan Lee・Sang Young Nam

Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju, 28130, Korea
Correspondence to: Y. S. Huh (⊠)
Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju, 28130, Korea
Received September 7, 2017; Revised October 12, 2017; Accepted October 12, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar ‘Cheongsu’ which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.
Keywords : Mulberry, Abscisic acid, Ex vitro transfer, Acclimatization, Water stress

December 2017, 44 (4)
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