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Conditions of In Vitro Spore Germination and Prothallium Culture for Sporophyte propagation of Polystichum braunii (Spenn.) Fée
J Plant Biotechnol 2017;44:454-461
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Hyuk Joon Kwon1・Ji Hyun Han2・Cheol Hee Lee2・Soo-Young Kim1

1National Institute of Biological Resources, Incheon 22689, Korea
2Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University
Correspondence to: S.-Y. Kim (⊠)
National Institute of Biological Resources, Incheon 22689, Korea
These authors contributed equally to this work.
Received July 26, 2017; Revised October 19, 2017; Accepted October 20, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation, and seedling growth in Polystichum braunii (Spenn.) Fée. The rate of spore germination and early prothalium development was high in Knop (41.2%), which had low mineral content. The optimal medium for prothallus propagation and sexual organ formation was 2MS medium (2% sucrose). Among the various mixtures of cultivation soil (bedding soil, peat moss, perlite, and decomposed granite), a mixture of bedding soil and decomposed granite at a ratio of 2:1 (v:v) had a positive effect on sporophyte formation (276.3 ea/7.5 m2). The most efficient conditions for promoting the growth of sporophytes were pots filled with only bedding soil.
Keywords : Braun’s holly fern, Fern, Masspropagation, Medium type, Prickly shield fern, Pteridophyta

December 2017, 44 (4)
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