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Genetic diversity and herbicide resistance of 15 Echinochloa crus-galli populations to quinclorac in Mekong Delta of Vietnam and Arkansas of United States
J Plant Biotechnol 2017;44:472-477
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Duy Le1・Chon M. Nguyen2・Richard K. Mann3・Carla N. Yerkes3・Bobba V. N. Kumar4

1Dow AgroSciences B.V 106 Nguyen Van Troi, Phu Nhuan district, Ho Chi Minh city, Vietnam.
2Can Tho University, Can Tho city, Vietnam
3Dow AgroSciences LLC, Indianapolis, U.S.
4Dow AgroSciences LLC, Kuala Lumpur, Malaysia
Correspondence to: D. Le (⊠)
Dow AgroSciences B.V 106 Nguyen Van Troi, Phu Nhuan district, Ho Chi Minh city, Vietnam.
e-mail: DLE@Dow.com
Received September 7, 2017; Revised September 28, 2017; Accepted September 29, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Barnyardgrass (Echinochloa crus-galli) is one of the worst weeds in rice (Oryza sativa), but there are few reports about the genetic diversity and herbicide resistance of barnyardgrass in Vietnam. In this study, we used random amplified polymorphic DNA (RAPD) analysis and greenhouse testing to study the genetic diversity and quinclorac resistance levels of 15 Echinochloa crus-galli populations in the Mekong Delta, Vietnam, and the state of Arkansas, U.S. The quinclorac resistance of Echinochloa crus-galli populations in Vietnam was confirmed; 9 populations were resistant to quinclorac with R/S ratios ranging from 1.9 to 6.3. Six oligonucleotide primers produced a total of 55 repeatable bands of which 46 were polymorphic (83.3% average) among the 15 populations. Genetic distance was calculated, and cluster analysis separated the 15 populations into 2 main clusters with the genetic distances within the clusters ranging from 0.09 to 0.39. The two main clusters were divided into 7 subclusters, and the quinclorac resistant and susceptible populations were located randomly within each subcluster. Six out of 13 weed populations from Vietnam belonged to one cluster and a single Echinochloa species. The remaining 7 populations were identified as potentially different species in the Echinochloa genus. Nine Echinochloa populations from Vietnam were tested and identified as quinclorac resistant. The connection between quinclorac resistance levels and weed groups defined by RAPD analysis in the study is unclear; the quinclorac resistance of each resistant population could have evolved individually, regardless of differences in genetic diversity and location of the sampled populations.
Keywords : Echinochloa, RAPD, Quinclorac, Herbicide Resistance, Rice


December 2017, 44 (4)
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