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Optimization of in vitro lily culture system with different treatments of taurine
J Plant Biotechnol 2017;44:484-489
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Sang-Hee Lee1・Hwan-Rae Yang1・Sun Tae Kim2・Tae Hwan Jun2・Yong Chul Kim2・Jong Bo Kim1

1Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university, Choong-Ju, 27478, Korea
2Department of Plant Bioscience, Pusan National University, Miryang, 627-706, South Korea
Correspondence to: J. B. Kim (⊠)
Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus. Konkuk university, Choong-Ju, 27478, Korea
e-mail: jbhee1011@kku.ac.kr
Contributed equally
Received December 6, 2017; Revised December 19, 2017; Accepted December 19, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning.

We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.

Keywords : Browning, lily, mass propagation, regeneration, taurine


December 2017, 44 (4)
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