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Establishment of optimal conditions for micropropagation by node culture and multiple shoots formation from sucker explants of thornless Blackberry (Rubus fruticosus L. cv. BB21)
J Plant Biotechnol 2018;45:110-116
Published online June 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Kang Seop Lee, Hyo Jin Kim*, Dae Hyun Park, Seung Cheol Oh, Han Jig Cho, Ee Youb Kim

Bioplus Co., LTD, 312, Changjogwan, 224, Wanjusandan 6-ro, Bongdong-eup, Wanju-gun 55315, Korea
Correspondence to: H. J. Kim (✉) e-mail: cattleya77@naver.com
These authors contributed equally to this work.
Received April 26, 2018; Revised June 12, 2018; Accepted June 12, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study was conducted to develop a simple, rapid, and reliable method for in vitro propagation of disease-free and true-to-type clones from sucker explants of thornless blackberry (Rubus fruticosus L. × R. parvifolius L.). To induce multiple shoots, the sucker explants were sterilized in 1% NaOCl solution, and then were aseptically cultured on the full and 1/2 MS solid medium supplemented with BAP (0.1, 0.5, 1.0, 2.0 mg/L). After six weeks of culture, the highest frequency (85.4%) of shoot formation from sucker explants was obtained on the full-strength MS medium with 1.0 mg/L BAP. Node explants obtained from multiple shoots were cultured on the various media of full- or half-strength of AD, B5, MS, SH, QL, WPM media, respectively. After 30 days of culture, plant growth was good on the half-AD, half-QL medium. After 90 days of culture, plant growth was good on the full MS and full SH medium. The survival rate of the plantlets after transfer to plastic pots containing soil mixture (sand: soil: vermiculite was 1:1:1, vol.) in the greenhouse was 98%. The results indicate that a multiple-shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. × R. parvifolius L.
Keywords : Blackberry, Node culture, multiple shoots, Sucker, Rubus fruticosus


June 2018, 45 (2)
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