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Effect of carbon source and concentration on in vitro regeneration and propagation in Lycopus lucidus by node culture
J Plant Biotechnol 2018;45:131-139
Published online June 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Na-Nyum Lee1†, Ji Ah Kim1†*, Yong Wook Kim2, Tae Dong Kim1

1Forest Biotechnology Division, National Institute of Forest Science (NIFoS), Suwon, 16631, Korea
2Special Forest Products Division , National Institute of Forest Science (NIFoS), Suwon, 16631, Korea
Correspondence to: J. A. Kim (✉) e-mail: jiahkim@korea.kr
These authors contributed equally to this work.
Received November 14, 2017; Revised March 26, 2018; Accepted April 9, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.
Keywords : Lycopus lucidus Turcz. ex Benth., Carbon source, Regeneration, Acclimatization


June 2018, 45 (2)
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