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Screening of melon genotypes identifies gummy stem blight resistance associated with Gsb1 resistant loci
J Plant Biotechnol 2018;45:217-227
Published online September 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Md Zahid Hassan, Arif Hasan Khan Robin, Md Abdur Rahim, Sathishkumar Natarajan, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou

Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of orea
Department of Agronomy, Patuakhali Science and Technology University, Dumki, Patuakhali 8602, Bangladesh
Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
University-Industry Cooperation Foundation, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea
Correspondence to: e-mail: htkim@sunchon.ac.kr, nis@sunchon.ac.kr
Received September 14, 2018; Revised September 14, 2018; Accepted September 20, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Gummy stem blight (GSB) is one of the most destructive and economically important, soil borne diseases of melon caused by the ascomycete fungus, Didymella bryoniae throughout the world. In Korea, however, no GSB resistant genotype has been reported yet. The study aimed to identify GSB resistant melon germplasm. We screened a total of 60 genotypes including 16 lines and 44 melon cultivars collected from USA and Korea. Among the 16 melon lines, four lines including ‘PI482399’, ‘PI140471’, ‘PI136170’ and ‘PI420145’, and two Korean cultivars viz. ‘Asia Papaya’ and ‘Supra’ showed complete resistance. We were aware that both genotypic and environmental variations could influence the phenotypic screening of resistance and susceptibility. We therefore, further assessed all genotypes using 20 SSR markers. The SSR marker ‘CMCT505’ linked to Gsb1 in chromosome 1 perfectly grouped resistant and susceptible lines indicating that resistance is probably due to the presence of Gsb1 gene. Cloning and sequencing of resistant and susceptible Gsb1 amplicons showed that there were 32-bp deletions in resistant line and 39-bp deletions in resistant cultivar compared to susceptible one. Thus, the resistant melon lines and cultivars identified in this study could be recommended for the melon breeding program. Furthermore, the SSR marker ‘CMCT505’ which is tightly linked with Gsb1 could be used for molecular screening of melon germplasm.
Keywords : Gummy stem blight, Didymella bryoniae, Melon, SSR markers, Screening


September 2018, 45 (3)
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