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Evaluating phylogenetic relationships in the Lilium family using the ITS marker
J Plant Biotechnol 2018;45:236-241
Published online September 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Sina Ghanbari, Barat Ali Fakheri, Mohammad Reza Naghavi, Nafiseh Mahdinezhad

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Plant Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
Correspondence to: e-mail: or
Received June 19, 2018; Revised July 23, 2018; Accepted July 23, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.
Keywords : Molecular phylogeny, Lilium, ITS marker, Haplotype

September 2018, 45 (3)
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