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Metabolic comparison between standard medicinal parts and their adventitious roots of Cynanchum wilfordii (Maxim.) Hemsl. using FT-IR spectroscopy after IBA and elicitor treatment
J Plant Biotechnol 2018;45:250-256
Published online September 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Myung Suk Ahn, Eun Jin So, Eun Yee Jie, So Yeon Choi, Sang Un Park, Byeong Cheol Moon, Young Min Kang, Sung Ran Min, Suk Weon Kim

Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea
Floriculture Research Division, National Institute of Horticultural and Herbal Science, Jeollabuk-do 55365, Republic of Korea
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil,
Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea
Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 34134, Republic of Korea
Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea
Correspondence to: e-mail: srmin@kribb.re.kr
e-mail: kimsw@kribb.re.kr
These authors contributed equally to this work.
Received July 19, 2018; Revised July 23, 2018; Accepted July 23, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To determine whether metabolite fingerprinting for whole cell extracts based on Fourier transform infrared spectroscopy (FT-IR) can be used to discriminate and compare metabolic equivalence, standard medicinal parts of Cynanchum wilfordii (Maxim.) Hemsl. and their adventitious roots were subjected to FT-IR. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from FT-IR spectral data showed that whole metabolic pattern from the adventitious root of Cynanchum wilfordii was highly similar to its standard medicinal parts. These results clearly showed that mass proliferation of adventitious roots could be applied for the novel supply of standard medicinal parts of medicinal plants. Furthermore, FT-IR spectroscopy combined with multivariate analysis established in this study could be applied as an alternative tool for discriminating of whole metabolic equivalence from standard medicinal parts. Thus, it is proposed that these metabolic discrimination systems from the adventitious root of Cynanchum wilfordii could be applied for metabolic standardization of in vitro grown Cynanchum wilfordii.
Keywords : Adventitious root, Cynanchum wilfordii (Maxim.) Hemsl., Fourier transform infrared spectroscopy (FT-IR), Partial least square discriminant analysis (PLS-DA), Principal component analysis (PCA)


September 2018, 45 (3)
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