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Callus induction and in vitro plant regeneration of Polygonatum stenophyllum Maxim.
J Plant Biotechnol 2018;45:266-272
Published online September 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Min Wan Park, Shi Hyun Ryu, Yoon Young Lee, Jae-Mo Song, Jin Ho Kim, Young-Hee Ahn, Kee Hwa Bae

Nakdonggang National Institute of Biological Resources, Sangju, 37242, Republic of Korea
Hwanghaksan Arboretum, Yeoju, 12653, Republic of Korea
Forest Science Institute of Gangwon Province, Chuncheon 24207, Republic of Korea
Kyungpook National University, Sangju, 37224, Republic of Korea
Department of Plant System Science, Chung-Ang University, Anseong 17546, Republic of Korea
Correspondence to: e-mail: khbae7724@nnibr.re.kr
Received August 10, 2018; Revised August 23, 2018; Accepted August 23, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A Polygonatum stenophyllum Maxim. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of P. stenophyllum through plant regeneration from rhizome (1-year, 3-years, and 5-years) explant-derived calli. The rhizome segments were cultured in Murashige and Skoog (MS) medium supplemented with varying concentrations of 2,4-D (0, 0.5, 1.0, 1.5 mg・L-1) for callus induction. In media supplemented with 0.5 mg・L-1 of 2,4-D, 87% of 3-years rhizome produced callus. Subsequently, the callus was transferred to 1/2MS medium supplemented with various concentrations of IAA, IBA, NAA, and 2,4-D (0, 0.1, 0.5 and 1.0 mg・L-1) for adventitious shoot formation. The highest percentage of adventitious shoot induction (57%) was observed in 1/2MS medium containing 0.5 mg・L-1 of NAA. Elongation of the adventitious shoot was achieved in 1/2MS medium supplemented with 0.1 mg・L-1 of BA. Rooting was achieved in 1/2MS medium without any hormones. It is hypothesized that the stated in vitro propagation protocol will be useful for conservation and mass propagation of the endangered Polygonatum stenophyllum Maxim. for bioresources.
Keywords : Adventitious shoot, Rhizome, Root induction


September 2018, 45 (3)
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