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Enhancement of eurycomanone biosynthesis in cell culture of longjack (Eurycoma longifolia) by elicitor treatment
J Plant Biotechnol 2018;45:340-346
Published online December 31, 2018
© 2018 The Korean Society for Plant Biotechnology.

Nguyen Huu Nhan, Nguyen Hoang Loc

Institute of Bioactive Compounds, College of Sciences, Hue University, Hue 530000, Vietnam
College of Food Industry, Danang 550000, Vietnam
Correspondence to: e-mail: nhloc@hueuni.edu.vn
Received September 6, 2018; Revised October 10, 2018; Accepted October 10, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In this study, the effect of elicitors such as yeast extract (YE), methyl jasmonate (MeJA) and salicylic acid (SA) on the accumulation of eurycomanone in Eurycoma longifolia cell cultures were investigated. Suspension cells of E. longifolia was cultured in Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 1.25 mg/L naphthaleneacetic acid (NAA) and 1 mg/L kinetin at a shaking speed of 120 rpm. Elicitors were added in the culture at different concentrations and times to stimulate eurycomanone accumulation in the Eurycoma longifolia cells. Eurycomanone content was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, and a detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitrile: H2O. Non-elicited cells were used as the control. The study showed the effect of different elicitor concentrations, YE at 200 mg/L, MeJA at 20 μM and SA at 20 μM stimulated high production of eurycomanone. In which, treatment of 20 μM MeJA after 4 days of culture resulted in the highest accumulation of this compound (17.36 mg/g dry weight), approximately 10-fold higher than that of untreated cells (1.70 mg/g dry weight).
Keywords : Elicitors, Eurycoma longifolia, eurycomanone, suspension cell


December 2018, 45 (4)
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