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Effect of growth regulators on In Vitro mass propagation of Haworthia maughanii
J Plant Biotechnol 2018;45:369-374
Published online December 31, 2018
© 2018 The Korean Society for Plant Biotechnology.

Youn Hee Kim, Hye Hyeong Kim, Gee Young Lee, Jae Hong Lee, Jae Hong Jung, Pablo Delgado-Sánchez, Sang Deok Lee

Cactus and Succulents Research Institute, Goyang-si, Gyeonggi-do, Republic of Korea
Horticultural Research Division, GASRES, Hwaseong, 18388, Korea
Botechnology Lab, Faculty of Agronomy and Veterinary, Universidad Autónoma de San Luis Potosí, Soledad de Graciano Sánchez, SLP., Mexico
Correspondence to: e-mail: sky3884@gg.go.kr
Received November 13, 2018; Revised December 11, 2018; Accepted December 12, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0)which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The purpose of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shoot and rooting for the in vitro mass production of Haworthia maughanii. To determine suitable parts of the plant for callus induction, the leaves, flower bloom and flower stalks were cultured in MS medium at different concentrations of 0 ~ 2 mgL-1 NAA and 0 ~ 2 mgL-1 TDZ, respectively. All of the parts showed 100% callus formation rate at NAA 1 mgL-1 and TDZ 1 mgL-1 treatment, NAA 2 mgL-1 and TDZ 2 mgL-1 treatment and NAA 1 to 2 mgL-1, respectively. While the rate of callus formation was high in all parts, the leaves were the most efficient to obtain most culture parts. NAA 0.1 mg L-1 and BA 0.1 mg L-1 treatments were the most effective in shoot formation with 22.0 shoots. In addition, multiple shoot propagation showed 16.3 shoots, the highest, with NAA 0.1 mg L-1 and BA 0.1 mg L-1 treatments. These results led us to speculate that the optimization of culture conditions was responsible for the mass propagation for in vitro cultures of Haworthia maughanii.
Keywords : Haworthia, Growth regulators, Propagation, Regenerated shoot, acclimatization


December 2018, 45 (4)
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