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Effect of plant growth regulators and carbon sources on proliferation and shoot formation of PLBs in Dendrobium candidum
J Plant Biotechnol 2019;46:9-16
Published online March 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Jee-woo Jang, Chang Kil Kim, Trinh Ngoc Ai, Do-Jin Lee and Mi Young Chung

Department of Agricultural Education, Sunchon National University, Suncheon 57922, Korea
Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
School of Agriculture and Aquaculture, Tra Vinh University, Trà Vinh, Vietnam
Correspondence to: e-mail: queen@sunchon.ac.kr
These authors contributed equally to this work.
Received November 5, 2018; Revised December 10, 2018; Accepted December 10, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocorm-like bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in H3P4 basal medium containing 0.1 mg · L-1 NAA and 0.1 mg · L-1 Kinetin. PLB growth was the best when 10 g · L-1 sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or H1P2 medium containing 10 g · L -1 sucrose, and the shoot length was longer than the others.
Keywords : Micro-propagation, H3P4 basal medium, Chinese medicine plant


March 2019, 46 (1)
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