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Effect of nitrogen sources and 2, 4-D treatment on indirect regeneration of ginger (Zingiber officinale Rosc.) using leaf base explants
J Plant Biotechnol 2019;46:17-21
Published online March 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Valiyaparambath Musfir Mehaboob, Kunnampalli Faizal, Palusamy Raja, Ganesan Thiagu, Abubakker Aslam and Appakan Shajahan

Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli 620020, India
Correspondence to: e-mail: shajahan.jmc@gmail.com
Received February 1, 2019; Revised March 2, 2019; Accepted March 2, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium (NH4+) and nitrate (NO3-) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number (7.33± 0.33) and length (88.33±4.40) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.
Keywords : Ginger, Callus, Ammonium, Nitrate, BA, Hardening


March 2019, 46 (1)
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