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Whole genome re-sequencing and development of SSR markers in oriental melon
J Plant Biotechnol 2019;46:71-78
Published online June 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Woon-Ho Song · Sang-Min Chung

Department of Life Science, Dongguk University-seoul 04620, Korea
Correspondence to: e-mail: smchung@dongguk.edu
Received April 9, 2019; Revised April 16, 2019; Accepted April 16, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of this study was to use ‘Danta PR’, NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, ‘Hyangseo 1’ and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of ‘Danta PR’ BC1 (F1 x ‘Danta PR’) population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.
Keywords : Indel, Oriental melon, Polymorphic markers, Resequencing, SSR


June 2019, 46 (2)
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Funding Information
  • Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries
      10.13039/501100003668
      317011-04-3-HD020
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