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Physiological and molecular analysis of OsTPS30 by gamma irradiation
J Plant Biotechnol 2019;46:88-96
Published online June 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Se Won Kim · In Jung Jung · Sang Hoon Kim · Hong-Il Choi · Si-Yong Kang · Jin-Baek Kim

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup, Jeollabuk 56212, Korea
Correspondence to: e-mail: jbkim74@kaeri.re.kr
Received February 1, 2019; Revised March 29, 2019; Accepted March 29, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Terpenes constitute a large class of secondary metabolites in plants. The Oryza sativa terpene synthase is a vital gene in plant defense response. In this study, the molecular and physiological functions of Oryza sativa terpene synthase 30 (OsTPS30, LOC_Os08g07080) were investigated after exposure of the seeds and plants to gamma-rays. The OsTPS30 expression was slightly induced at 200 Gray (Gy), but was significantly induced at 400 Gy. The total terpenoid was synthesized more in OsTPS30- overexpressing (OX-OsTPS30) Arabidopsis thaliana plants than in wild-type (WT) plants. The OX-OsTPS30 plants exhibited resistance to gamma-rays, as compared to WT. The OX-OsTPS30 plants had significantly increased height and weight after gamma irradiation. Additionally, the activity of antioxidant enzymes was increased more in OX OsTPS30 plants than in WT plants after gamma irradiation. Furthermore, the OsTPS30-GFP fusion protein was mostly localized in the chloroplast, suggesting that OsTPS30 is putative MEP pathway-related terpene synthase.
Keywords : Terpene synthase, gamma-ray, chloroplast, MEP pathway


June 2019, 46 (2)
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