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In vitro mass propagation and acclimatization of Haworthia truncata
J Plant Biotechnol 2019;46:127-135
Published online June 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Youn Hee Kim · Gee Young Lee · Hye Hyeong Kim · Jae Hong Lee · Jae Hong Jung · Sang Deok Lee

Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services, Goyang, 10224, Korea,
Horticultural Research Division, Gyeonggi-Do Agricultural Research and Extension Services, Hwaseong, 18388, Korea
Correspondence to: e-mail : sky3884@gg.go.kr
Received April 13, 2019; Revised May 10, 2019; Accepted May 16, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The purpose of this study was to investigate suitable parts for callus induction and optimal concentrations of growth regulators, contained in the medium affecting shoot and rooting for in vitro mass production of Haworthia truncata. Leaves and flower bud showed 100% callus formation rate at NAA 1 ~ 2 mgL-1 treatment, and NAA 1 mgL-1 + TDZ 2 mgL-1 treatment. The flower stalk showed 75% callus formation rate, at NAA 2 mgL-1 + TDZ 2 mgL-1 treatment in H. truncata. While the rate of callus formation was high in leaves and flower bud, leaves were the most efficient in obtaining most culture parts. Shoot induction rate from callus was highest, at NAA 0.1 mgL-1 treatment in H. truncata. Additionally, the number of shoots formation was 66.3 shoots high, in NAA 1 mg L-1 + BA 0.1 mgL-1 treatment in H. truncata. In the case of acclimatization of regenerated plant, growth characteristics did not show significant difference (95%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate, considering plant height and appearance preference of H. truncata. It was established that optimization of culture condition, was responsible for mass propagation in vitro cultures of H. truncata.
Keywords : Haworthia, Growth regulators, Propagation, Regenerated shoot, Acclimatization


June 2019, 46 (2)
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