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Isolation and characterization of Auxin/indole-3-acetic acid 1 (Aux/IAA1) gene from poplar (Populus alba × P. glandulosa)
J Plant Biotechnol 2019;46:180-188
Published online September 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Eun-Kyung Bae ·Young-Im Choi ·Hyoshin Lee ·Ji Won Choi

Forest Biotechnology Division, National Institute of Forest Science, Suwon, 16631, Korea
Forest Bioinformation Division, National Institute of Forest Science, Suwon, 16631, Korea
Correspondence to: e-mail: baeek@korea.kr
Received August 10, 2019; Revised September 9, 2019; Accepted September 9, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Auxin plays a crucial regulatory role in plant growth and development processes. Three major classes of auxin-responsive transcription factors controlled by the Auxin/indole-3-acetic acid (Aux/IAA), Gretchen Hagen 3 (GH3), and small auxin up RNA (SAUR) genes regulate auxin signaling. Aux/IAA, in particular, encodes short-lived nuclear proteins that accumulate rapidly in response to auxin signaling. In this study, we isolated a PagAux/IAA1 gene from poplar (Populus alba × P. glandulosa) and investigated its expression characteristics. The PagAux/IAA1 cDNA codes for putative 200 amino acids polypeptide containing four conserved domains and two nuclear localization signals (NLSs). Utilizing Southern blot analysis, we confirmed that a single copy of the PagAux/IAA1 gene was present in the poplar genome. The expression of this gene is specific to leaves and flowers of the poplar. PagAux/IAA1 expressed in the early exponential growth phase of cell-cultured in suspension. PagAux/IAA1 expression level reduced in drought and salt stress conditions, and the presence of plant hormones such as abscisic acid. However, expression enhanced in cold stress, cambial cell division, and presence of plant hormones such as gibberellic acid and jasmonic acid. Thus, these results suggest that PagAux/IAA1 participates in cold stress response as well as developmental processes in the poplar.
Keywords : Abiotic stress, auxin, cambial cells, PagAux/IAA1, Populus alba × P. glandulosa, suspension cell culture


September 2019, 46 (3)
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