
Large functional genomics initiatives have revealed that many human disorders are affected by multiple genes and their genetic and molecular interactions with each other and the environment. Thus, the use of various model systems such as human and mammalian cell lines, yeast (i.e.,
Recent reports have shown that
Alzheimer’s disease (AD) is the most common age-related neurodegenerative disorder of the central nervous system (Mattson 2004), which is characterized by the deposition of aggregation-prone amyloid-β peptides (Aβs) as the degraded products of the amyloid precursor protein (APP) and neurofibrillary tangles in the brain (Selkoe 1998). In humans, presenilin (PS) was identified from the genetic screens of patients suffering from AD and mutations in
APP as one of more than 80 substrates for γ-secretase is a metazoan protein, which is only present in genomes of multicellular animals (Haapasalo and Kovacs 2011). However, the components of γ-secretase have been identified in both metazoan and plants (Grigorenko et al. 2002; Kimberly and Wolfe 2003; Moliaka et al. 2004; Ponting et al. 2002; Wigge and Weigel 2001). The moss
Here we presented the effect of 42 amino acids long- human amyloid-β peptide (hAβ42) on
The wild-type [ecotype Columbia (Col-0)] and the transgenic
For treatment of 42 amino acids long-human amyloid-β peptide (hAβ42) at cellular level, we followed the procedures for isolation of
The procedures for were previously described (Dahlgren et al. 2002; Kook et al. 2012). hAβ42 peptide was dissolved at a concentration of 1 mg・ml-1 in 3,3,3-Hexafluoro-2- propanol (HFIP) (Sigma-Aldrich). HFIP was evaporated using Speed Vacuum concentrator and lyophilized peptide was resolved in 10 ml DMSO. After calculation of the molar concentration, aliquots of 1 mM lyophilized hAβ42 monomer were stored at -80°C until use.
For
Because the accumulation of aggregated form of Aβ42 in the brain leads to mitochondrial dysfunction and elevated reactive oxygen species (ROS), consequently resulting in cell death (Reddy and Beal 2008), we investigated whether hAβ42 monomer affects the viability of plant cells. Mesophyll protoplasts were isolated form 10-d-old
Because hAβ42 monomer affects the viability of
In order to investigate the effect of the hAβ42 overexpression in
Here we provide the first data on the effect of hAβ42 monomer as a metazoan protein in angiosperms. In a series of experiments, we showed that the viability of cells was reduced in hAβ42 monomer-treated
We thank Dr. Mook-Jung (Seoul National University) for kindly providing research methods. This work was supported by National University Promotion Program in 2019 (to J.H. Lee).
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