Intraspecific genetic variation in Corynandra chelidonii (Angiosperms: Cleomaceae) as revealed by SCoT, ISSR and RAPD analyses
Subash Sirangi ･Phanikanth Jogam･Gandhi Nemali ･Ragan Ajmeera ･Sadanandam Abbagani ･Vatsavaya S. Raju
Department of Botany, Kakatiya University, Warangal, Telangana - 506009, India
Department of Biotechnology, Kakatiya University, Warangal, Telangana - 506009, India
Department of Biotechnology, Kakatiya University, Warangal, Telangana - 506009, India
Correspondence to: e-mail: rajuvatsavaya@gmail.com
†Subash Sirangi and Phanikanth Jogam equally contributed to this work.
†Subash Sirangi and Phanikanth Jogam equally contributed to this work.
Received July 7, 2020; Revised August 23, 2020; Accepted August 24, 2020.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.- Abstract
- The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii
- Keywords : Cleome chelidonii, varieties, genetic variation analyses, molecular markers, taxonomic status assessment
- December 2020, 47 (4)
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