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A novel technique for recombinant protein expression in duckweed (Spirodela polyrhiza) turions
J Plant Biotechnol 2021;48:156-164
Published online September 30, 2021
© 2021 The Korean Society for Plant Biotechnology.

Salil Chanroj · Aompilin Jaiprasert · Nipatha Issaro

Department of Biotechnology, Faculty of Sciences, Burapha University, Chonburi, 20131 Thailand
Department of Biotechnology, Faculty of Sciences, Burapha University, Chonburi, 20131 Thailand
Division of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, 20131 Thailand
Correspondence to: e-mail: nipatha@go.buu.ac.th
Received July 13, 2021; Revised August 17, 2021; Accepted August 17, 2021.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Spirodela polyrhiza, from the Lenmaceae family, are small aquatic plants that offer an alternative plant-based system for the expression of recombinant proteins. However, no turion transformation protocol has been established in this species. In this study, we exploited a pB7YWG2 vector harboring the eYFP gene that encodes enhanced yellow fluorescent protein (eYFP), which has been extensively used as a reporter and marker to visualize recombinant protein localization in plants. We adopted Agrobacterium tumefaciens mediated turion transformation via vacuum infiltration to deliver the eYFP gene to turions, special vegetative forms produced by duckweeds to endure harsh conditions. Transgenic turions regenerated several duckweed fronds that exhibited yellow fluorescent emissions under a fluorescence microscope. Western blotting verified the expression of the e YFP protein. To the best of our knowledge, this is the first report of an efficient protocol for generating transgenic S. polyrhiza expressing e YFP via Agrobacterium tumefaciens mediated turion transformation. The ability of turions to withstand harsh conditions increases the portability and versatility of transgenic duckweeds, favoring their use in the further development of therapeutic compounds in plants.
Keywords : Agrobacterium tunefaciens-mediated turion transformation, Enhanced yellow fluorescent protein, Fluorescence microscopy, Spirodela polyrhiza, Turion, Vacuum infiltration


September 2021, 48 (3)
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