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  • Research Article 2024-04-22

    0 277 45

    In Silico identification of dengue NS5 phytochemical inhibitors as potential antiviral drug compounds via molecular docking

    Earl Adriane Cano ・Jovito San Luis III ・Julia Cassandra Perez ・Aleezah Priela ・Eiby Grace Ramos ・ Mhizzy Reyes ・Luis Antonio Rico ・LJ Sabado

    J Plant Biotechnol (2024) 51:100-110

    https://doi.org/10.5010/JPB.2024.51.011.100

    Abstract

    Abstract : Dengue fever is a viral disease caused by the dengue flavivirus, transmitted through the bites of infected mosquitoes. The endeavor to combat dengue has led many researchers to develop antiviral drugs. Several medicinal plants, containing diverse phytochemicals, exhibit potential for inhibiting pathogenic proteins and are employed in ongoing research and therapeutic advancement. This has piqued researchers’ interest in identifying potential plant-derived molecule inhibitors 』of the dengue virus Non-Structural Protein 5 (NS5) and analyzing their subsequent interactions. This study employed candidate selection and molecular docking using an in silico approach. We used the Protein Data Bank database and performed National Center for Biotechnology Information (NCBI) blastp analysis to gather and compare the best protein structures of the two NS5 serotypes. In addition, we identified ligand molecules previously reported to exhibit potential inhibitory effects against NS5 by retrieving their 3D structures from NCBI PubChem. Moreover, we utilized PyRx and PyMOL to perform molecular docking. The findings revealed a conspicuous prevalence of interactions between the MTase domain in NS5 and phytochemical compounds, notably Kaempferol and 6-Shogaol. Based on the evidence presented in this study, we propose that investigating the NS5 protein in flavivirus is necessary for dengue fever prevention, as these proteins play a vital role in the replication of the virus. Our findings provide valuable insights beneficial for advancing research into antiviral medication. We suggest further investigation into other medicinal plants that may inhibit dengue and the additional scrutiny of Kaempferol and 6-Shogaol, the compounds that yielded significant results in this study

  • Research Article 2024-04-16

    0 129 30

    Evaluating the insecticidal potential of ethanol extracts from Melia azedarach Linn. against Bactrocera cucurbitae - a pest inflicting damage on Momordica charantia Linn.

    Tran Thi Phuong Nhung ・Le Pham Tan Quoc

    J Plant Biotechnol (2024) 51:089-099

    https://doi.org/10.5010/JPB.2024.51.010.089

    Abstract

    Abstract : This study explores the insecticidal efficacy of ethanol extracts - obtained from the fruits and seeds of Melia azedarach (MAFS) - against Bactrocera cucurbitae. We assessed the effectiveness of the MAFS extracts at concentrations ranging from 1 to 625 ppm using both laboratory and greenhouse models. Sofri protein 10 DD (1.2 liters/ha) served as the standard insecticide, while water functioned as the negative control. Key parameters evaluated include pupation period, germination time, quantities of pupae and adult individuals, and the severity of damage to bitter melon fruits. In the laboratory model, MAFS significantly prolonged the pupation period (p < 0.05), reduced pupal numbers (p < 0.05), and affected the pupation percentage of B. cucurbitae (p < 0.05). In addition, the germination time (p < 0.05) and proportion of adult B. cucurbitae emergence (p < 0.05) were also significantly impacted. In the greenhouse experiment, MAFS significantly reduced the quantity of B. cucurbitae eggs on bitter melon plants (p < 0.05), resulting in a notable decrease in both larval (p < 0.05) and pupal quantities (p < 0.05). The inhibitory effects of MAFS on larval (p < 0.05) and pupal quantities (p < 0.05), as well as survival from the larval to adult stage, were equivalent to the sofri protein (p < 0.05). MAFS effectively mitigated the severity of damage to bitter melon fruits caused by B. cucurbitae (p < 0.05). Moreover, MAFS exhibits significant effects throughout the various developmental stages of B. cucurbitae. These findings support the potential of MAFS ethanol extracts as an efficient and eco-friendly solution for pest infestation management.

  • Review 2024-04-09

    0 218 41

    Industrial interests and requirements for improvement of pearl millet, a gluten-free and climate-smart underutilized cereal

    Leila Riahi ・Mériam Ben-Romdhane ・Ahmed S. Masmoudi

    J Plant Biotechnol (2024) 51:077-088

    https://doi.org/10.5010/JPB.2024.51.009.077

    Abstract

    Abstract : Pearl millet is a small-seeded staple crop in arid and semi-arid regions of Africa and Asia. It is the crop of choice in the hottest and driest climates where other cereals do not grow well and is considered a future climate-smart cereal. This underutilized cereal provides nutritional and food security to millions of people. It is also used as animal feed, fuel, and fodder as well as in brewing. The gluten-free grains of this cereal are rich sources of fats, proteins, carbohydrates, minerals, especially iron and zinc, and phenolics with health-promoting properties. Pearl millet also has industrial uses in the production of bioenergy, biodegradable products, bio-coagulants, and construction materials, and in tissue engineering. Pearl millet hybrids have gained considerable popularity among Indian farmers, resulting in a significant increase in production yields. However, pearl millet production in Africa is primarily dependent on traditional landraces with limited acceptance of improved open-pollinated varieties and hybrids. Consequently, no significant increase in pearl millet production has been achieved over the past few decades. Despite its inherent resilience against poor climates, this staple crop faces a complex array of abiotic and biotic stresses in its production areas, which is exacerbated by ongoing climate change. Furthermore, certain anti-nutritional traits impede its overall nutritional value and effective utilization. Hence, improving pearl millet is a continuous and significant challenge for plant breeders and biotechnologists.

  • Research Article 2024-04-08

    0 162 39

    Genome-wide identification of oleosin family and expression analysis in response to abiotic stresses in balloon flower (Platycodon grandiflorus)

    Eunhui Kim・Tae Kyung Hyun

    J Plant Biotechnol (2024) 51:071-076

    https://doi.org/10.5010/JPB.2024.51.008.071

    Abstract

    Abstract : Oleosins (OLEs) are structural proteins commonly found within oil bodies (OBs), playing a significant role in regulating the number, size, and stability of OBs. Therefore, this study aims to comprehensively analyze the OLE family in balloon flower (Platycodon grandiflorus) - a significant medicinal plant. Through genome-wide exploration and bioinformatics analyses, we identified and characterized five putative OLE proteins from P. grandiflorus (PlgOLEs). This study provides a comprehensive overview of this gene family in balloon flowers, including phylogenic analysis, conserved motifs, isoelectric points, and hydrophobicity. The study revealed the presence of central hydrophobic regions with a proline knot motif, a characteristic feature shared by OLE proteins in various plant species. Additionally, tissue-specific expression analysis revealed that PlgOLEs were predominantly expressed in seeds, indicating their crucial role in seed OB stability. Furthermore, expression profiling under abiotic stresses demonstrated that PlgOLEs are stress-inducible genes, suggesting their diverse physiological functions in stress responses. These findings shed light on the functional diversity of OLEs in balloon flowers and establish a basis for further research, including genetic modification studies, to elucidate their physiological roles.

  • Research Article 2024-03-28

    0 225 45

    Effects of flooding conditions and phytohormones on coleoptile elongation in Indica rice varieties

    Khac Nhu Nghi ・Ngoc Ai Trinh

    J Plant Biotechnol (2024) 51:063-070

    https://doi.org/10.5010/JPB.2024.51.007.063

    Abstract

    Abstract : The unpredictable scenarios of current climate change pose great challenges for global food security. Climate change triggers submergence, a natural disaster that affects cereal production in the Mekong Delta of Vietnam. Among cereals, rice is a unique species in its ability to germinate and develop a long coleoptile under water. Several previous studies indicated that the capacity of japonica rice is better than indica rice cultivars. Herein, we assessed the ability of coleoptile elongation and the role of exogenous auxin (IAA) in promoting rice coleoptile length under dark, flooded conditions using 20 indica rice varieties collected from the Mekong Delta. We germinated 20 rice seeds per variety under water (with and without adding auxin at different concentrations) for several days and measured the coleoptile length at two different timepoints of submergence. We found that the ability to develop a long coleoptile under water was not related to the rice subspecies. The average coleoptile length at day 8 and the growth curve from the beginning to day 8 of the submergence experiment of our rice cultivars were the same. We also found that 10 μM of IAA concentration expressed a significant effect on the capacity of coleoptile elongation in both long and short coleoptile-harboring indica rice subspecies. Our results suggested that OM108, OM5451, and ĐT8 are promising rice cultivars for rice production in the Mekong Delta of Vietnam.

  • Research Article 2024-03-07

    0 277 92

    Best practices for initiation of banana and plantain (Musa spp.) cultures

    Fatma H. Kiruwa ・Emanuel Epathra Mlinga ・Agatha Amnaay Aloyce ・Mpoki Mathew Shimwela

    J Plant Biotechnol (2024) 51:055-062

    https://doi.org/10.5010/JPB.2024.51.006.055

    Abstract

    Abstract : Banana is a staple food and income crop worldwide. Here, we identified the best practices for the initiation of banana (Musa spp.) cultures. The cultivars studied included East African highland bananas (Mshare/Mchare and Nshakara) and plantains (Mzuzu), which are highly demanded by farmers. The following factors were assessed: sterilization techniques for rainy and dry seasons, explant size, effect of benzylaminopurine (BAP) concentration on regeneration of cultures, effect of ascorbic acid on culture browning, and culturing conditions. We found that treatment with ethanol alone (70%) or in combination with sodium hypochlorite (1%) yielded a relatively higher proportion (≥ 96%) of aseptic culture establishment in both rainy and dry seasons. Explant size affected the survival and regeneration of cultures where small explants (< 10 mm) like meristem dome showed relatively slower re-growth and high mortality in contrast to large explants (≥ 10 mm). Moreover, culturing in BAP-supplemented Murashige and Skoog medium (MS) significantly enhanced the greening/shoot regeneration percentage at 14 d. However, there was no significant increase in number of green shoots/culture regeneration at BAP concentrations of 5 mg/l, 6 mg/l, and 8 mg/l for Nshakara and 3 mg/l, 5 mg/l, and 6 mg/l for Mzuzu. Browning decreased by 18% when explants were pre-treated with 100 mg/l ascorbic acid for 10 min. Further, growth conditions such as light were a relatively greater determinant for regeneration of initiated banana cultures. Therefore, our findings suggest the use of appropriate sterilization techniques, explant size, growth regulators, and conditions to ensure sufficient production of planting materials.

  • Research Article 2024-03-05

    0 253 42

    Chemical profile and antimicrobial activity of Ocimum gratissimum L. essential oil from Dak Lak province, Vietnam

    Pham My Hao ・Le Pham Tan Quoc

    J Plant Biotechnol (2024) 51:050-054

    https://doi.org/10.5010/JPB.2024.51.005.050

    Abstract

    Abstract : Essential oils (EOs) are predominantly found in odorous plants, particularly in Mediterranean and tropical countries worldwide. These oils hold significant value as crucial components in traditional medicine systems. Moreover, they are widely used in food technology, medicine, and cosmetics. EOs of different origins may have unique properties. Therefore, this study aimed to design and analyze the chemical composition and antimicrobial activity of the EO extracted from Ocimum gratissimum L., sourced from Dak Lak province (Vietnam). The chemical composition of the EO was assessed using gas chromatography-mass spectrometry. The results showed 23 compounds, with eugenol accounting for the highest proportion (76.01%) and its EO exhibiting potent antimicrobial activity against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus, as assessed via the agar disc diffusion method. Therefore, the EO extracted from O. gratissimum can be considered a natural antibacterial and aromatic agent suitable for application in the pharmaceutical and food industries.

  • Research Article 2024-02-19

    0 309 58

    Transcriptome analysis of a tropical medicinal plant, Pistacia weinmannifolia

    Mi Kyung Choi ・Bimpe Suliyat Azeez ・Sang Woo Lee ・Wan Yi Li ・Sangho Choi ・Ik-Young Choi ・Ki-Young Choi ・Jong-Kuk Na

    J Plant Biotechnol (2024) 51:033-049

    https://doi.org/10.5010/JPB.2024.51.004.033

    Abstract

    Abstract : Pistacia weinmannifolia has long been used as an herbal medicine for treating various illnesses. The genomic information of P. weinmannifolia will help elucidate the chemical constituents that exert medicinal effects; however, genomic studies have rarely been performed. Therefore, we conducted a transcriptome analysis of P. weinmannifolia using the Illumina RNA sequencing system. We obtained a total of 18 million high-quality paired-end reads with 2,230 Mbp. De novo assembly of high-quality reads generated a total of 18,956 non-redundant contigs with an average length of 901 bp, of which 18,296 contigs (96.5%) were annotated. The total length of all unigenes was 17,080,830 bp, and the GC content and N50 were 43.2% and 1,137 bp, respectively. Annotation using The Kyoto Encyclopedia of Genes and Genomes (KEGG) assigned a total of 5,095 unigenes (26.9%), of which 3,166 were mapped to 410 different KEGG metabolic pathways. A comparison of unigenes between P. weinmannifolia and Pistacia chinensis showed that 8,825 unigenes were highly similar to each other. Simple sequence repeats were mined, and valuable data for further comparative and functional genomic studies were obtained to uncover the mechanisms underlying the medicinal properties of P. weinmannifolia as an important medicinal plant. Several genes of P.weinmannifolia involved in the biosynthesis of eugenol and isoeugenol were also identified in this study.

  • Research Article 2024-01-25

    1 473 103

    Effects of plant hormones on the characteristics and the genetic stability of calli induced from the ex vitro tissues of Celosia argentea var cristata

    Nhat-Anh Tran-Nguyen・My Y Huynh・Hong Hanh Doan・Phuong Ngo Diem Quach・Thanh-Hao Nguyen・ Vi An Ly

    J Plant Biotechnol (2024) 51:024-032

    https://doi.org/10.5010/JPB.2024.51.003.024

    Abstract

    Abstract : Celosia argentea var cristata, commonly known as the cockscomb plant, is a popular ornamental species in Vietnam. Its propagation primarily relies on seeds, enabling widespread cultivation but leading to a notable absence of micropropagation research in the country. This practice poses a potential threat to preserving unique traits susceptible to loss through segregation. To address this gap, this study focused on the impact of plant hormones on callus formation in various aerial tissues - leaves, stems, and newly emerging inflorescences - gathered from plants grown on soil. The calli displayed distinct morphological characteristics under the influence of different combinations of 6-Benzyladenine (BAP), 1-Naphthaleneacetic acid (NAA), and 2,4-Dicholorophenoxyacetic acid (2,4-D). Furthermore, we investigated the genetic stability of C. argentea var cristata calli using random amplified polymorphic DNA (RAPD) markers. The calli persistently cultured on medium containing 2 mg/L BAP and 2 mg/L NAA maintained their genetics stability, as assessed through four RAPD markers: OPA-13, OPA-15, OPA-18 (G), and OPD-2.

  • Research Article 2024-01-23

    1 301 76

    Assessment of the antioxidant and nematicidal activities of an aqueous extract of Chromolaena odorata (L.) King and Robins against Radopholus similis infestation in Cavendish banana plants: An in vitro and in vivo study

    Tran Thi Phuong Nhung・Le Pham Tan Quoc

    J Plant Biotechnol (2024) 51:011-023

    https://doi.org/10.5010/JPB.2024.51.002.011

    Abstract

    Abstract : Here, we investigated the antioxidant and nematicidal activities of the aqueous leaf and stem extract of Chromolaena odorata (L.) (AECO) against Radopholus similis, a nematode pest of banana plants. In vitro antioxidant analysis involved testing AECO at concentrations ranging from 50 to 300 μg/mL in 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging assays. Both in vitro and in vivo experiments were performed using doses of 780, 1,560, 3,120, 6,250, and 12,500 mg DW/L AECO. We assessed the egg hatching inhibition and juvenile survival rate of R. similis, content of antioxidant compounds in banana roots, dry weight of the aerial parts and roots, and the nematode density in the soil. In vitro antioxidant assays revealed substantial DPPH-scavenging (59.67-92.13%) and ABTS radical inhibition (37.26% at 300 μg/mL) activities. In vitro experiments using 12,500 mg DW/L AECO exhibited significant inhibition (p < 0.05) of R. similis egg hatching (26.98%, 55.25%, and 82.92% at 24, 48, and 72 h, respectively) and reduced juvenile survival (p < 0.05). In vivo experiments demonstrated a significant decrease (p < 0.05) in malondialdehyde concentration and an increase (p < 0.05) in antioxidant production (glutathione, catalase, and superoxide dismutase) in banana roots after AECO treatment. Plant biomass showed significant differences (p < 0.05), with the highest values (15.38 ± 0.13 g the aerial part dry weight and 29.32 ± 0.15 g the root dry weight) recorded in the AECO12500 treatment. Notably, R. similis density was significantly decreased (p < 0.05) in the soil after AECO treatment, with maximum inhibition obtained using 12,500 mg/kg. These findings emphasize the potential of AECO for pest management and its relevance to the cultivation of Cavendish bananas.

  • Research Article 2024-01-12

    1 362 82

    Overexpression of ZjWRKY10, a Zoysia japonica WRKY transcription factor gene, accelerates leaf senescence and flowering in transgenic Arabidopsis

    Yueyue Yuan・Ji-Hi Son・Mi-Young Park・Hyeon-Jin Sun・Hyo-Yeon Lee・Hong-Gyu Kang

    J Plant Biotechnol (2024) 51:001-010

    https://doi.org/10.5010/JPB.2024.51.001.001

    Abstract

    Abstract : The WRKY transcription factors play an important role in plants’ stress response, leaf senescence, growth, and development. In this study, we cloned ZjWRKY10 from the leaf of Korean lawngrass (Zoysia japonica), a warm-season turf; the deduced protein sequence showed high homology with the TaWRKY10 protein of wheat. The ZjWRKY10 and TaWRKY10 genes belong to group IIc of the WRKY transcription factor family, which regulates tolerance to multiple abiotic stresses. The study’s results showed that ZjWRKY10 was slightly upregulated by cold, sodium chloride, and polyethylene glycol 6000 treatments; however, it was strongly activated by a dark treatment. When ZjWRKY10 was overexpressed in Arabidopsis thaliana after dark treatment, it resulted in earlier leaf senescence compared with wild-type plants. In addition, the transgenic plants overexpressing ZjWRKY10 showed early-flowering phenotypes when exposed to long-day conditions compared with the wild-type plants. When comparing the transgenic with the wild-type plants, the increased expression of the FLOWERING LOCUS T (FT) gene, vital in triggering flowering, supported the earlier flowering observed in the transgenic Arabidopsis plants. These results support that ZjWRKY10 may be involved in the regulation of leaf senescence and flowering.

JPB
Vol 51. 2024

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eISSN 2384-1397
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