Journal of

Plant Biotechnology

Assembly result of the complete chloroplast genome sequence of S. brevicaule (MK036507). Four representative contigs of the chloroplast genome of S. brevicaule and comparison with the corresponding regions of the chloroplast genome of S. berthaultii (KY419708; Kim et al. 2018) are combined. Blue and yellow bars indicate contig matching with the reference sequence in forward and reverse orientations, respectively|@|~(^,^)~|@|Gene map of the chloroplast genome of S. brevicaule. Genes on the outside and inside of the map are transcribed in the clockwise and counterclockwise directions, respectively|@|~(^,^)~|@|Maximum-likelihood phylogenetic tree of S. brevicaule with 11 species belonging to the Solanaceae family based on the chloroplast protein-coding sequences of each chloroplast genome. The numbers on each node indicate the bootstrap values from 1,000 replicates. The figure has been partially adopted from Park (2019)|@|~(^,^)~|@|Multiple alignment of the sequences on the intergenic or intragenic regions containing SNPs used to develop the PCR-based markers. SA11, SB2-1, SD9, Stub, Scmr, Sblb, Scha, and Ssto indicate the chloroplast genome sequences of S. acaule (MK036506; Park 2021a), S. brevicaule (MK036507; In this study), S. demissum (MK036508; Cho et al. 2019), S. tuberosum (KM489056; Cho et al. 2016), S. commersonii (KM489054; Cho et al. 2016), S. bulbocastanum (DQ347958; Daniell et al. 2006), S. chacoense (MF471371; Kim and Park 2019), and S. stoloniferum (MF471373; Kim and Park 2020a), respectively. They are listed from top to bottom in each region of the SNPs. The regions of the SNPs detected on that of S. brevicaule are highlighted and underlined. Restriction sites are indicated in red and bold font|@|~(^,^)~|@|PCR-based markers for the discrimination of S. brevicaule from other Solanum species. A: SB2_SNP_3. B: SB2_SNP_5. C: SB2_SNP_8. The three markers all generated bands positively and/or negatively specific to S. brevicaule. Red and yellow allows indicate positive and negative bands on S. brevicaule, respectively. SB2-1 and SB2-17 indicate two different lines of S. brevicaule (PI205394). M, SH, HR, DJ, PT56, SA, SB1, SD, SC4, SC1, SC2, SI, SK, SJ, SM2, SM1, SP, SS, SV2, and SV1 indicate a size marker ladder, the potato varieties ‘Seohong’, ‘Haryeong’, ‘Daeji’, a potato breeding line ‘PT56’, S. acaule (PI310970), S. berthaultii (PI310981), S. demissum (PI218047), S. candolleanum (PI210035), S. cardiophyllum (PI341233), S. commersonii (PI558050), S. iopetalum (PI230459), S. kurtzianum (PI578236), S. jamesii (PI578326), S. microdontum (PI310979), S. mochiquense (PI338616), S. pinnatisectum (PI190115), S. stoloniferum (PI160224), S. vernei (PI230468) and S. verrucosum (PI160228), respectively

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