Journal of

Plant Biotechnology

Result of assembling the complete chloroplast genome sequence of S. demissum. The four representative contigs for the chloroplast genome of S. demissum and comparison with the corresponding regions of the S. berthaultii chloroplast genome (KY419708) are combined. Blue and yellow bars indicate contig matching with the reference sequence in forward and reverse orientations, respectively|@|~(^,^)~|@|Gene map of the S. demissum chloroplast genome. Genes on the outside of the map are transcribed in the clockwise direction, and genes on the inside of the map are transcribed in the counterclockwise direction|@|~(^,^)~|@|Maximum-likelihood phylogenetic tree of S. demissum with eleven Solanaceae family members based on the chloroplast protein-coding sequences of each chloroplast genome. The numbers on each node indicate the bootstrap values from 1,000 replicates|@|~(^,^)~|@|Multiple alignment of the sequences on the intergenic regions containing InDels used to develop the PCR-based markers. The chloroplast genome sequences of S. acaule (SA11: MK036506), S. brevicaule (SB2-1: MK036507), S. demissum (SD9: MK036508), S. tuberosum (Stub: KM489056), S. commersonii (Scmr: KM489054), S. bulbocastanum (Sblb: DQ347958), S. chacoense (Scha: MF471371), and S. stoloniferum (Ssto: MF471373) were used and are listed from top to bottom in each region of the InDels. The regions of the InDels detected on that of S. demissum are bold and highlighted|@|~(^,^)~|@|PCR-based markers for the discrimination of S. demissum from other Solanum species. A: SD9_InDel_1. B: SD9_InDel_2. The two markers are all positively specific to S. demissum. SD7 and SD9 indicate two different lines of S. demissum (PI218047). M is a size marker ladder. SH, HR, DJ, SA, SB1, SB2, SC4, SC1, SC3, SC2, SI, SK, SJ, SM2, SM1, SP, SS, SV2, and SV1 indicate potato varieties ‘Seohong’, ‘Haryeong’, ‘Daeji’, S. acaule (PI310970), S. berthaultii (PI310981), S. brevicaule (PI205394), S. candolleanum (PI210035), S. cardiophyllum (PI341233), S. chacoense (PI201846), S. commersonii (PI558050), S. iopetalum (PI230459), S. kurtzianum (PI578236), S. jamesii (PI578326), S. microdontum (PI310979), S. mochiquense (PI338616), S. pinnatisectum (PI190115), S. stoloniferum (PI160224), S. vernei (PI230468) and S. verrucosum (PI160228), respectively

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