Journal of

Plant Biotechnology

The induction of shoots from apical buds of Tilia mandshurica on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP) after 6 weeks of culture. Apical bud explants were taken from 5-month-old seedlings (A) and 12-year-old trees (B)|@|~(^,^)~|@|The rooting of Tilia mandshurica shoots in vitro that were cultured in half-strength Murashige and Skoog (MS) medium supplemented with various concentrations of indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) after 3 weeks of culture|@|~(^,^)~|@|The acclimatization of Tilia mandshurica plantlets that were rooted on half-strength Murashige and Skoog (MS) medium supplemented with or without auxin (IBA or NAA). (A) Plantlets transplanted in plastic containers with soil. (B) Plants acclimatized in plastic containers with soil for 5 weeks. (C) Comparison of the growth of plantlets rooted on auxin (IBA or NAA)-supplemented and auxin-free media after 5 weeks of acclimatization. Scale bar = 5 cm. (D) Cultivation of potted plants in the greenhouse

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