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  • ReviewSeptember 30, 2014

    0 118 1053

    Production of vegetable oil in biomass

    Hyun Uk Kim, Kyeong-Ryeol Lee, Eun-Ha Kim, Kyung Hee Roh, Han Chul Kang, Jong-Bum Kim

    J Plant Biotechnol 2014; 41(3): 107-115

    Abstract

    Abstract : Vegetable oils (triacylglycerols) produced mainly in seeds of plants are used for valuable foods that supply essential fatty acids for humans as well as industrial raw materials and biofuel production. As the demanding for vegetable oils has increased, plant metabolic engineering to produce triacylglycerols in biomass such as leaves has been considered and explored for alternative source of vegetable oils. Leaves are genetically programmed to supply the fixed carbon by photosynthesis to other organs for plant development and growth. Therefore, in order to produce and accumulate triacylglycerols in leaves, one should take account of multiple metabolic pathways such as carbon flux, competition of carbohydrate and fatty acid biosynthesis, and triacylglycerols turnover in leaves. The recent metabolic engineering strategy has showed potential in which the co-expression of three genes WRINKLED1, DGAT1, and OLEOSIN involved in the critical step for increasing the fatty acid synthesis, accumulating triacylglycerols, and protecting triacylglycerols, respectively produced higher amount of vegetable oils in leaves. Developing of genetically engineered plants producing vegetable oil in biomass at non-agricultural lands will be promising to the future success of the field.

  • Research ArticleSeptember 30, 2014

    0 145 833

    Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions

    Mi-Suk Seo, Seong-Han Sohn, Beom-Seok Park, Ho-Cheol Ko, Mina Jin

    J Plant Biotechnol 2014; 41(3): 116-122

    Abstract

    Abstract : Total of fifty accessions of Brassica rapa with various morphological characteristics were used for production of double haploid plants though microspore culture in Brassica rapa. Among them, only 30 accessions induced embryos from microspores. The highest efficiency of embryo induction of 1.194 per bud was obtained from IT135449 of turnip type, while 3 accessions of sarson (winter oil) type did not generate embryo. The effect of heat shock periods for embryogenesis was also investigated with 4 accessions (IT135449; Turnip type, IT199710; Chinese cabbage type, IT212886; Pak choi type, IT218043; Summer oil type). The high productions of embryos were observed in IT135449, IT199710 and IT212886 when microspores were pre-cultured to 32˚C for 2 days. In IT218043, high embryogenesis was observed at the 3 days of heat shock treatment. The optimal condition of shoot regeneration for IT199710 was observed in MS medium supplemented with NAA 0.5 mg·L-1 and BAP 1 mg·L-1. In contrast, the IT135449 and IT212886 were observed high regeneration frequency in MS medium without plant growth regulators. All the plantlets regenerated from microspore-derived embryos have been successfully transplanted to soil, and bud self-pollinated seeds were produced from doubled haploid plants. This indicated that double-haploid genotype was likely generated naturally during embryogenesis process.

  • Research ArticleSeptember 30, 2014

    0 74 1185

    LED array design for optimal combination of plant grown

    Sungwon Lee, Sekwang Park

    J Plant Biotechnol 2014; 41(3): 123-126

    Abstract

    Abstract : This paper is suitable for household plant factory by design and using both energy-saving LED and solar technology. Conventional household plant factory only depending on natural sunlight is sensitive for the change of external environment. Another a big problem of conventional common household plant factory is large power consumption. Recently interest in wellbeing food such as chemical-free is increased abruptly. To solve these two problems, this paper describes hybrid type of household plant. In particular, reducing the power photosynthesis photon flux density (PPFD) is kept uniform to enhance the growth of the plant. Ambient light sensor is adopted for the control of proper combination of sunlight and LED to keep PPFD constant.

  • Research ArticleSeptember 30, 2014

    0 108 554

    Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice

    Jin-Hyoung Lee, Kong-Sik Shin, Seok-Cheol Suh, Seong-Lyul Rhim, Myung-Ho Lim, Hee-Jong Woo, Yang Qin, Soon-Jong Kweon, Soon-Ki Park

    J Plant Biotechnol 2014; 41(3): 127-133

    Abstract

    Abstract : Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

  • Research ArticleSeptember 30, 2014

    0 142 715

    Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings

    Eun-Heui Han, Yong-Wan Son, Man-Bae Kim, Yong-Wook Shin, Young-Son Cho, Shin-Woo Lee

    J Plant Biotechnol 2014; 41(3): 134-139

    Abstract

    Abstract : The aim of this study was to establish the condition of regeneration for white balloon flower (Platycodon grandiflorum DC. cv. Jangback) and to manage for the raising of seedling with in vitro regenerated plants. It was examined that 0.5 mg/L of NAA and 1.0 mg/L of BA was the best composition for the callus and shoot induction (up to 600%). NAA was better than IBA for the induction of root and it took 16.9 days for the induction of rooting on the MS soild media containing 0.5 mg/L of NAA and the final rooting ratio was up to 75%. Out of 5 different bed soils purchased from local market, “Tosil” was identified to be the best for the acclimation and growth of in vitro regenerated balloon flower. In detail, on 8 weeks after planting of in vitro regenerated plants in pots containing “Tosil” bed soils, the plant hight was increased up to 2-fold (12.8 cm), 3.5-fold (27) for the number of leaf and 1.5-fold (4.5 cm) for the leaf length when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to prevent the occurrence of blue balloon flower in the massive cultivated area of white balloon flower by providing the seedlings raised from in vitro regenerated plants.

  • Research ArticleSeptember 30, 2014

    0 87 518

    Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.)

    Soo-Yun Kim, Yul-Kyun Ahn, Yun-Chan Huh, Hye-Eun Lee, Do-Sun Kim

    J Plant Biotechnol 2014; 41(3): 140-145

    Abstract

    Abstract : This study were carried out for selection of proper transformation variety and development of efficient regeneration and transformation methods. The number of shoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dish containing on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistant pure lines were wide variation on the deviation. The expression of GFP was high 67% and 100% at the co-cultivation with Agrobacterium. The effective shoot regeneration plant hormone were combination BA and 2,4-D. The number and elongation condition of shoot was good after 4 weeks change with MS medium supplemented with 1 mg/L BA. Effective callus production plant hormone were combination of 3 mg/L BA and 0.1 mg/L 2.4-D.

  • Research ArticleSeptember 30, 2014

    0 59 610

    Isolation and functional analysis of three microsomal delta-12 fatty acid desaturase genes from Camelina sativa (L.) cv. CAME

    Hyojin Kim, Young Sam Go, Augustine Yonghwi Kim, Sanghyeob Lee, Kyung-Nam Kim, Geung-Joo Lee, Gi-Jun Kim, Mi Chung Suh

    J Plant Biotechnol 2014; 41(3): 146-158

  • Research ArticleSeptember 30, 2014

    0 93 570

    Characterization of peptide:N-glycanase from tomato (Solanum lycopersicum) fruits

    Soo Jin Wi, Ky Young Park

    J Plant Biotechnol 2014; 41(3): 159-167

    Abstract

    Abstract : In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.

JPB
Vol 51. 2024

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Plant Biotechnology

pISSN 1229-2818
eISSN 2384-1397
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