The effect of selective media on frond regeneration and the transformation efficiency of S. polyrhiza turions. Co-cultivation represents
Selective media
Co-cultivation
Total number of turions
Number of transformed cells
Transformation efficiency (%)
MS2
16 h light/8 h dark
10
10
90.00±8.16A,#,##
10
8
10
9
Dark
10
8
83.33±4.71*,**
10
8
10
9
MS3
16 h light/8 h dark
10
6
50.00±8.16A
10
5
10
4
Dark
10
5
30.00±16.33
10
3
10
1
Each cultivation group consisted of 10 turions (n = 10). Percentage transformation efficiency is expressed as mean ± standard deviation (SD) of three replicates. Uppercase letters (A) indicate no significant difference between the 16 h light/8 h dark and dark co-cultivation conditions on the same selective medium. The symbol # indicates significant differences on the selective MS2 medium cultivated under 16 h light/8 h dark (# p < 0.05; ## p < 0.01) compared with selective MS3 medium cultivated under 16 h light/8 h dark and dark co-cultivation conditions, respectively. The symbol * indicates significant differences on the selective MS2 medium cultivated under dark (*p < 0.05; **p < 0.01) compared with selective MS3 medium cultivated under 16 h light/8 h dark and dark co-cultivation conditions, respectively.