Journal of

Plant Biotechnology

Fig. 1. In vitro multiplication and tuberous root induction in Holostemma annulare. (a) Shoot initiation in Murashige and Skoog (MS) medium supplemented with 0.25 mg/l 6-benzyl adenine (BA). (b) Shoot multiplication in ½ MS medium supplemented with 0.25 mg/l (BA). (c) Shoot initiation in Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg/l BA. (d) Shoot multiplication in SH medium containing 2.0 mg/l BA in combination with 0.5 mg/l kinetin and 1.0 mg/l indole 3-butyric acid. (e) and (f) Tuberous root induction from leaf and shoots, respectively, in SH medium fortified with 0.5 mg/l α-naphthaleneacetic acid. (g) Tuberous roots after 32 days of culture. (h) Root biomass in root suspension culture. (i) Shoot initiation from the in vitro tuberous roots in suspension culture

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