Journal of
Plant Biotechnology

Table 1

Effect of plant growth regulators in Schenk and Hildebrandt medium on shoot culture initiation in Holostemma annulare

BA	Kinetin	NAA	Number of shoots		Length of shoots (cm)		Number of nodes per shoots

			Node	Shoot tip	Node	Shoot tip	Node	Shoot tip
0.25	-	-	1.88 ± 0.01^b	1.83 ± 0.44^b	11.91 ± 0.01^b	10.21 ± 0.21^b	5.51 ± 0.31^b	5.83 ± 0.10^b
0.5	-	-	1.95 ± 0.12^a	1.92 ± 0.21^a	10.82 ± 0.02^c	10.01 ± 0.43^b	6.31 ± 0.31^a	6.16 ± 0.05^a
1.0	-	-	1.45 ± 0.37^d	1.56 ± 0.42^e	7.38 ± 0.01^f	8.21 ± 0.31^d	5.91 ± 0.43^b	5.71 ± 0.03^b
2.0	-	-	1.34 ± 0.36^e	1.36 ± 0.16^f	6.21 ± 0.01^g	6.41 ± 0.36^f	5.12 ± 0.22^b	5.13 ± 0.11^b
0.25	0.5	-	1.67 ± 0.43^c	1.66 ± 0.21^d	10.52 ± 0.02^c	9.22 ± 0.41^c	3.92 ± 0.32^e	2.63 ± 0.22^e
0.5	0.5	-	1.24 ± 0.32^f	1.77 ± 0.43^c	8.23 ± 0.03^e	8.03 ± 0.26^d	3.22 ± 0.23^e	3.34 ± 0.04^d
1.0	0.5	-	1.13 ± 0.04^g	1.15 ± 0.09^g	6.24 ± 0.04^g	6.14 ± 0.13^f	3.22 ± 0.22^e	3.23 ± 0.13^d
2.0	0.5	-	1.04 ± 0.08^h	1.05 ± 0.05^h	4.03 ± 0.02^h	3.92 ± 0.02^g	2.82 ± 0.41^f	2.74 ± 0.33^e
0.25	-	0.1	1.97 ± 0.21^a	1.95 ± 0.03^a	13.72 ± 0.02^a	12.83 ± 0.13^a	6.52 ± 0.28^a	6.43 ± 0.42^a
0.5	-	0.5	1.85 ± 0.42^b	1.73 ± 0.07^c	9.52 ± 0.02^d	9.33 ± 0.23^c	4.34 ± 0.34^d	4.23 ± 0.17^c
1.0	-	1.0	1.64 ± 0.11^c	1.54 ± 0.20^e	8.43 ± 0.03^e	8.23 ± 0.25^d	4.12 ± 0.13^d	4.33 ± 0.21^c
2.0	-	1.5	1.22 ± 0.32^f	1.34 ± 0.72^f	7.93 ± 0.03^f	7.53 ± 0.28^e	3.22 ± 0.15^e	3.32 ± 0.32^d

BA - 6-benzyl adenine; NAA - α-naphthaleneacetic acid

Data represents the mean ± SE of ten replicates, repeated thrice. Mean values followed by the same superscript letter do not differ significantly based on the ANOVA and Duncan’s multiple range test (p ≤ 0.05).

J Plant Biotechnol 2022;49:230~239 https://doi.org/10.5010/JPB.2022.49.3.230

Journal ofPlant Biotechnology

Journal of
Plant Biotechnology