Journal of

Plant Biotechnology

Fig. 1. MAPK signaling inhibits the proliferation of nodule meristem cells during nitrogen-fixing nodule development. (A) U0126 treatment results in the formation of slightly large nodules in M. truncatula. The phenotypes of nodules on M. truncatula roots infected with Sinorhizobium meliloti for 3 weeks with/without 5 μM U0126. All inoculated S. meliloti strains containing a hemA-lacZ reporter gene for visualizing bacterial cells appear as blue in the β-galactosidase assay. (B) Microscopy analysis for the characterization of symbiotic nodules induced in the absence (left) or presence (right) of 5 μM U0126. Two-micrometer sections of 7-day-old nodules are shown. Sections of nodule zone I–II and zone III–IV are presented. Scale bars = 500 μm (A) and 200 μm (B)

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