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Fig. 6. DNA products were amplified by gradient PCR for ADS, CYP, and ALDH1 genes. Annealing temperatures for the gradient PCR were as follows: A) 75°C, B) 73.9°C, C) 72.1°C, D) 69.2°C, E) 65.5°C, F) 63°C, G) 61.1°C, and H) 60°C. The analyzed fragment sizes of the target genes were as expected: 94 bp for ADS, 157 bp for ALDH1, and 107 bp for CYP. The fragment sizes were calculated based on the 1kb DNA ladder marker
J Plant Biotechnol 2022;49:307~315
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