On-line First

J Plant Biotechnol

Published online May 10, 2023

© The Korean Society of Plant Biotechnology

Effect of carbon sources on somatic embryogenesis and cotyledon number variations in carrot (Daucus carrot L.)

Young Jin Lee 1, Kyu Seog Hwang 2, Pil Son Choi 1*

1Department of Emergency Medical Rescue, Nambu University, Gwangju 506–824, Korea , 2Department of Biomedicine Enginerring, Nambu University, Gwangju 506–824, Korea

Received: April 6, 2023; Revised: May 10, 2023; Accepted: May 10, 2023

Abstract

In order to investigate the effect of carbon sources on somatic embryogenesis and cotyledon number variations in carrot, embryogenic callus were cultured in the medium supplemented with sucrose or glucose, and with combination of 2% sucrose and various concentrations of mannitol or sorbitol. The maximum number of somatic embryos formed per flask (1,555.70) was obtained in the medium supplemented with 2% sucrose rather than glucose alone or a combination of manitol or sorbitol and 2% sucrose, and the number of somatic embryos was decreased with the increasing of manitol or sorbitol concentration. The frequencies of somatic embryos with two cotyledons were 35.14% for sucrose, 19.88% for glucose, 32.55% for manitol, and 38.59% for sorbitol, respectively, and the frequencies of abnormal somatic embryos having 3 or more cotyledons were 64.86% for sucrose, 80.12% for glucose, 67.44% for manitol, and 61.41% for sorbitol, respectively. Particularly, the frequency of somatic embryos with two cotyledons (59.16%) was the highest in the 2% sucrose medium compared to the frequency of abnormal somatic embryogenesis with three or more cotyledons, and the frequency gradually decreased with increasing concentration of glucose, manitol or sorbitol. According to these results, it was found that the ratio of abnormal somatic embryo was higher than the normal somatic embryo in carrot, and was shown that somatic embryogenesis and the cotyledon number was affected by the concentrations of sucrose, glucose as carbon source, and manitol and sorbitol as osmotic agents in culture medium.

Keywords carbohydrates, carrot, cotyledon morphology, osmotic agents, somatic embryogenesis

Article

On-line First

J Plant Biotechnol

Published online May 10, 2023

Copyright © The Korean Society of Plant Biotechnology.

Effect of carbon sources on somatic embryogenesis and cotyledon number variations in carrot (Daucus carrot L.)

Young Jin Lee 1, Kyu Seog Hwang 2, Pil Son Choi 1*

1Department of Emergency Medical Rescue, Nambu University, Gwangju 506–824, Korea , 2Department of Biomedicine Enginerring, Nambu University, Gwangju 506–824, Korea

Received: April 6, 2023; Revised: May 10, 2023; Accepted: May 10, 2023

Abstract

In order to investigate the effect of carbon sources on somatic embryogenesis and cotyledon number variations in carrot, embryogenic callus were cultured in the medium supplemented with sucrose or glucose, and with combination of 2% sucrose and various concentrations of mannitol or sorbitol. The maximum number of somatic embryos formed per flask (1,555.70) was obtained in the medium supplemented with 2% sucrose rather than glucose alone or a combination of manitol or sorbitol and 2% sucrose, and the number of somatic embryos was decreased with the increasing of manitol or sorbitol concentration. The frequencies of somatic embryos with two cotyledons were 35.14% for sucrose, 19.88% for glucose, 32.55% for manitol, and 38.59% for sorbitol, respectively, and the frequencies of abnormal somatic embryos having 3 or more cotyledons were 64.86% for sucrose, 80.12% for glucose, 67.44% for manitol, and 61.41% for sorbitol, respectively. Particularly, the frequency of somatic embryos with two cotyledons (59.16%) was the highest in the 2% sucrose medium compared to the frequency of abnormal somatic embryogenesis with three or more cotyledons, and the frequency gradually decreased with increasing concentration of glucose, manitol or sorbitol. According to these results, it was found that the ratio of abnormal somatic embryo was higher than the normal somatic embryo in carrot, and was shown that somatic embryogenesis and the cotyledon number was affected by the concentrations of sucrose, glucose as carbon source, and manitol and sorbitol as osmotic agents in culture medium.

Keywords: carbohydrates, carrot, cotyledon morphology, osmotic agents, somatic embryogenesis

JPB
Mar 16, 2023 Vol.50, pp. 1~88

Stats or Metrics

Share this article on

  • line
  • mail

Related articles in JPB

Journal of

Plant Biotechnology

pISSN 1229-2818
eISSN 2384-1397
qr-code Download