On-line First

J Plant Biotechnol

Published online May 11, 2023

© The Korean Society of Plant Biotechnology

Effects of Plant Preservative MixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its implementation in orchid conservation

Chanh Trung Tran 1*, Huy Tan Nguyen 1, Ha Thu Nguyen 1, Khanh Le 2, Hoang Huu Nguyen 1

1Research Center for High-Tech Application in Agriculture (RCHAA) – University of Science - Vietnam National University, Ho Chi Minh City., 2Cisbay Global Inc. - 6389 San Ignacio Ave San Jose, California 95119 – United States of America

Received: April 15, 2023; Revised: May 11, 2023; Accepted: May 11, 2023

Abstract

In vitro conservation is one the most effective solutions for rare plant protection, especially for orchid species. To maximize in vitro explant establishment success rates (Stage I) in conservation programs, the usage of enhancement solutions such as the Plant Preservative MixtureTM (PPMTM) should be highlighted. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model to evaluate PPMTM’s phytotoxicity to epiphytic orchid meristematic tissues. We found no observable inhibitory effect on protocorms, shoot, or root development when PPMTM was supplemented at 0.1%. Supplementation of PPMTM caused adverse effects on D. thyrsiflorum explants at levels above 0.2%. At high concentrations, young in vitro seedlings were damaged, especially on the root tissue. From this model, media supplemented with 0.1-0.2% PPMTM was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

Keywords protocorm, micropropogation, agriculture, production, Nervilia

Article

On-line First

J Plant Biotechnol

Published online May 11, 2023

Copyright © The Korean Society of Plant Biotechnology.

Effects of Plant Preservative MixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its implementation in orchid conservation

Chanh Trung Tran 1*, Huy Tan Nguyen 1, Ha Thu Nguyen 1, Khanh Le 2, Hoang Huu Nguyen 1

1Research Center for High-Tech Application in Agriculture (RCHAA) – University of Science - Vietnam National University, Ho Chi Minh City., 2Cisbay Global Inc. - 6389 San Ignacio Ave San Jose, California 95119 – United States of America

Received: April 15, 2023; Revised: May 11, 2023; Accepted: May 11, 2023

Abstract

In vitro conservation is one the most effective solutions for rare plant protection, especially for orchid species. To maximize in vitro explant establishment success rates (Stage I) in conservation programs, the usage of enhancement solutions such as the Plant Preservative MixtureTM (PPMTM) should be highlighted. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model to evaluate PPMTM’s phytotoxicity to epiphytic orchid meristematic tissues. We found no observable inhibitory effect on protocorms, shoot, or root development when PPMTM was supplemented at 0.1%. Supplementation of PPMTM caused adverse effects on D. thyrsiflorum explants at levels above 0.2%. At high concentrations, young in vitro seedlings were damaged, especially on the root tissue. From this model, media supplemented with 0.1-0.2% PPMTM was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

Keywords: protocorm, micropropogation, agriculture, production, Nervilia

JPB
Mar 16, 2023 Vol.50, pp. 1~88

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Plant Biotechnology

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eISSN 2384-1397
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