The Korean Society of Plant Biotechnology
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J Plant Biotechnol
Published online May 11, 2023
© The Korean Society of Plant Biotechnology
Effects of Plant Preservative MixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its implementation in orchid conservation
In vitro conservation is one the most effective solutions for rare plant protection, especially for orchid species. To maximize in vitro explant establishment success rates (Stage I) in conservation programs, the usage of enhancement solutions such as the Plant Preservative MixtureTM (PPMTM) should be highlighted. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model to evaluate PPMTM’s phytotoxicity to epiphytic orchid meristematic tissues. We found no observable inhibitory effect on protocorms, shoot, or root development when PPMTM was supplemented at 0.1%. Supplementation of PPMTM caused adverse effects on D. thyrsiflorum explants at levels above 0.2%. At high concentrations, young in vitro seedlings were damaged, especially on the root tissue. From this model, media supplemented with 0.1-0.2% PPMTM was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.
Keywords protocorm, micropropogation, agriculture, production, Nervilia
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On-line First
J Plant Biotechnol
Published online May 11, 2023
Copyright © The Korean Society of Plant Biotechnology.
Effects of Plant Preservative MixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its implementation in orchid conservation
Chanh Trung Tran 1*, Huy Tan Nguyen 1, Ha Thu Nguyen 1, Khanh Le 2, Hoang Huu Nguyen 1
1Research Center for High-Tech Application in Agriculture (RCHAA) – University of Science - Vietnam National University, Ho Chi Minh City., 2Cisbay Global Inc. - 6389 San Ignacio Ave San Jose, California 95119 – United States of America
Abstract
In vitro conservation is one the most effective solutions for rare plant protection, especially for orchid species. To maximize in vitro explant establishment success rates (Stage I) in conservation programs, the usage of enhancement solutions such as the Plant Preservative MixtureTM (PPMTM) should be highlighted. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model to evaluate PPMTM’s phytotoxicity to epiphytic orchid meristematic tissues. We found no observable inhibitory effect on protocorms, shoot, or root development when PPMTM was supplemented at 0.1%. Supplementation of PPMTM caused adverse effects on D. thyrsiflorum explants at levels above 0.2%. At high concentrations, young in vitro seedlings were damaged, especially on the root tissue. From this model, media supplemented with 0.1-0.2% PPMTM was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.
Keywords: protocorm, micropropogation, agriculture, production, Nervilia
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