On-line First

J Plant Biotechnol

Published online December 6, 2023

© The Korean Society of Plant Biotechnology

Coconut water: A new and promising method for improving the ability of recalcitrant crops to grow in tissue culture

Gideon Sadikiel Mmbando *

The University of Dodoma

Received: 13 September 2023; Revised: 6 December 2023; Accepted: 6 December 2023

Abstract

Genetic transformation provided a novel biotechnology to increase agricultural production and thus ensure food security challenged by global population growth and climate change. Important steps in the genetic modification of crops include the initiation and regeneration of embryogenic calluses. The majority of crops, however, are resistant to a variety of in vitro treatments, which impede genetic improvement. Many growth regulators required in in vitro culture media are also expensive. A low-cost and effective natural product is required to enhance these crops' in vitro tissue culture. The addition of coconut water (CW) to tissue culture media has been shown to be an inexpensive way to promote callus initiation and improve tissue culture efficiency. However, recent research on the application of CW to improve tissue culture capabilities is scarce. This review focuses on the numerous recent uses of CW for improving the capacity of various crop species to grow tissue cultures, its functions as an antioxidant, and other potential biotechnology uses. The knowledge presented here will be crucial in creating protocols for the effective transformation of recalcitrant crops, boosting their productivity and yield.

Keywords Coconut water, Tissue culture, Transformation, Regeneration, Callus induction

Article

On-line First

J Plant Biotechnol

Published online December 6, 2023

Copyright © The Korean Society of Plant Biotechnology.

Coconut water: A new and promising method for improving the ability of recalcitrant crops to grow in tissue culture

Gideon Sadikiel Mmbando *

The University of Dodoma

Received: 13 September 2023; Revised: 6 December 2023; Accepted: 6 December 2023

Abstract

Genetic transformation provided a novel biotechnology to increase agricultural production and thus ensure food security challenged by global population growth and climate change. Important steps in the genetic modification of crops include the initiation and regeneration of embryogenic calluses. The majority of crops, however, are resistant to a variety of in vitro treatments, which impede genetic improvement. Many growth regulators required in in vitro culture media are also expensive. A low-cost and effective natural product is required to enhance these crops' in vitro tissue culture. The addition of coconut water (CW) to tissue culture media has been shown to be an inexpensive way to promote callus initiation and improve tissue culture efficiency. However, recent research on the application of CW to improve tissue culture capabilities is scarce. This review focuses on the numerous recent uses of CW for improving the capacity of various crop species to grow tissue cultures, its functions as an antioxidant, and other potential biotechnology uses. The knowledge presented here will be crucial in creating protocols for the effective transformation of recalcitrant crops, boosting their productivity and yield.

Keywords: Coconut water, Tissue culture, Transformation, Regeneration, Callus induction

JPB
Vol 51. 2024

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Plant Biotechnology

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