On-line First

J Plant Biotechnol

Published online May 6, 2024

© The Korean Society of Plant Biotechnology

Completion of chloroplast genome sequence and development of PCR-based markers for Solanum pinnatisectum

Seoyeon Son 1, Tae-Ho Park 1*

Daegu University

Received: 18 April 2024; Accepted: 6 May 2024

Abstract

Solanum pinnatisectum is one of the wild potato species originating from Mexico. The species has been used for potato breeding due to resistance to several pathogens and insects such as Phytophthora infestans, Erwinia carotovorum, Colorado potato beetle, and Potato tuber moth. However, S. pinnatisectum is diploid and has an EBN value of one, which causes the sexual reproduction barriers between the species and cultivated potatoes. To overcome this and to introgress the novel traits from S. pinnatisectum into cultivated potatoes, the method of cell fusion can be used. In this study, the chloroplast genome sequence of S. pinnatisectum completed by next-generation sequencing technology (NGS) was described and compared with those of ten other Solanum species to develop S. pinnatisectum-specific PCR markers. The length of the complete chloroplast genome of S. pinnatisectum is 155,597bp and the structural organization of the chloroplast genome is similar to those of other Solanum species. Sequence alignment of the complete chloroplast sequences of eleven Solanum species including S. pinnatisectum identified 216 SNPs and 26 InDels specific to S. pinnatisectum. Based on these InDels and SNPs, six PCR-based markers were developed to identify the plastid genotype of S. pinnatisectum. The results obtained in this study will be used to distinguish S. pinnatisectum from other Solanum species, to select suitable genotypes in potato breeding using somatic hybridization, and to accelerate potato breeding using S. pinnatisectum.

Keywords cpDNA, InDels, PCR-based marker, Potato, SNPs, Solanum pinnatisectum

Article

On-line First

J Plant Biotechnol

Published online May 6, 2024

Copyright © The Korean Society of Plant Biotechnology.

Completion of chloroplast genome sequence and development of PCR-based markers for Solanum pinnatisectum

Seoyeon Son 1, Tae-Ho Park 1*

Daegu University

Received: 18 April 2024; Accepted: 6 May 2024

Abstract

Solanum pinnatisectum is one of the wild potato species originating from Mexico. The species has been used for potato breeding due to resistance to several pathogens and insects such as Phytophthora infestans, Erwinia carotovorum, Colorado potato beetle, and Potato tuber moth. However, S. pinnatisectum is diploid and has an EBN value of one, which causes the sexual reproduction barriers between the species and cultivated potatoes. To overcome this and to introgress the novel traits from S. pinnatisectum into cultivated potatoes, the method of cell fusion can be used. In this study, the chloroplast genome sequence of S. pinnatisectum completed by next-generation sequencing technology (NGS) was described and compared with those of ten other Solanum species to develop S. pinnatisectum-specific PCR markers. The length of the complete chloroplast genome of S. pinnatisectum is 155,597bp and the structural organization of the chloroplast genome is similar to those of other Solanum species. Sequence alignment of the complete chloroplast sequences of eleven Solanum species including S. pinnatisectum identified 216 SNPs and 26 InDels specific to S. pinnatisectum. Based on these InDels and SNPs, six PCR-based markers were developed to identify the plastid genotype of S. pinnatisectum. The results obtained in this study will be used to distinguish S. pinnatisectum from other Solanum species, to select suitable genotypes in potato breeding using somatic hybridization, and to accelerate potato breeding using S. pinnatisectum.

Keywords: cpDNA, InDels, PCR-based marker, Potato, SNPs, Solanum pinnatisectum

Journal of

Plant Biotechnology

pISSN 1229-2818
eISSN 2384-1397
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