On-line First

J Plant Biotechnol

Published online July 6, 2024

© The Korean Society of Plant Biotechnology

Effect of Silver Nanoparticles and Antioxidants on Micropropagation of Rosa hybrida ‘Sahara’ by Nodal Culture

Jung Won Shin 1, Sejinj Kim 2, Jin Hyun Choi 3, Changgil Kim 1*

1Kyungbuk National University, 2Protected Horticulture Research Institute, National Institute of Horticultural Herbal Science, 3Department of Advanced Organic Materials Science and Engineering, Kyungpook National University

Received: 12 June 2024; Accepted: 6 July 2024

Abstract

Excessive ethylene production in rose tissue culture is a major challenge, impacting rose health and cultivation. We aimed to investigate the effects of silver nitrate (AgNO3), silver nanoparticles (AgNPs), and antioxidants on plant growth and ethylene production to establish an in vitro growth method for Korean-cultivated Rosa hybrida ‘Sahara.’ Shoots were cultivated via nodal explant culture in a medium containing AgNPs, AgNO3, or antioxidants (ascorbic or citric acid or both). The study assessed the impact on growth, ethylene production, reactive oxygen species levels, and the expression of genes associated with ethylene biosynthesis and signal transduction. Adding AgNPs and AgNO3 to the medium mitigated overhydration and chlorosis, leading to improved SPAD values, fresh weight, and growth parameters compared to the control. Additionally, the AgNPs treatment group exhibited lower superoxide anion levels compared to all other treatment groups (p < 0.05). Ethylene concentrations and ethylene biosynthetic gene expression levels were significantly lower in the AgNPs and AgNO3 treatment groups than in the control group (p < 0.05). In contrast, antioxidant treatments showed significant effects. These results indicate that AgNPs hold promise for enhancing the quality of plantlets in rose tissue culture by mitigating ethylene-related challenges.

Keywords AgNPs (silver nanoparticles), Antioxidants, Ethylene production, in vitro∙micropropagation, Nodal culture, Rosa hybrida

Article

On-line First

J Plant Biotechnol

Published online July 6, 2024

Copyright © The Korean Society of Plant Biotechnology.

Effect of Silver Nanoparticles and Antioxidants on Micropropagation of Rosa hybrida ‘Sahara’ by Nodal Culture

Jung Won Shin 1, Sejinj Kim 2, Jin Hyun Choi 3, Changgil Kim 1*

1Kyungbuk National University, 2Protected Horticulture Research Institute, National Institute of Horticultural Herbal Science, 3Department of Advanced Organic Materials Science and Engineering, Kyungpook National University

Received: 12 June 2024; Accepted: 6 July 2024

Abstract

Excessive ethylene production in rose tissue culture is a major challenge, impacting rose health and cultivation. We aimed to investigate the effects of silver nitrate (AgNO3), silver nanoparticles (AgNPs), and antioxidants on plant growth and ethylene production to establish an in vitro growth method for Korean-cultivated Rosa hybrida ‘Sahara.’ Shoots were cultivated via nodal explant culture in a medium containing AgNPs, AgNO3, or antioxidants (ascorbic or citric acid or both). The study assessed the impact on growth, ethylene production, reactive oxygen species levels, and the expression of genes associated with ethylene biosynthesis and signal transduction. Adding AgNPs and AgNO3 to the medium mitigated overhydration and chlorosis, leading to improved SPAD values, fresh weight, and growth parameters compared to the control. Additionally, the AgNPs treatment group exhibited lower superoxide anion levels compared to all other treatment groups (p < 0.05). Ethylene concentrations and ethylene biosynthetic gene expression levels were significantly lower in the AgNPs and AgNO3 treatment groups than in the control group (p < 0.05). In contrast, antioxidant treatments showed significant effects. These results indicate that AgNPs hold promise for enhancing the quality of plantlets in rose tissue culture by mitigating ethylene-related challenges.

Keywords: AgNPs (silver nanoparticles), Antioxidants, Ethylene production, in vitro∙micropropagation, Nodal culture, Rosa hybrida

JPB
Vol 51. 2024

Stats or Metrics

Share this article on

  • line

Related articles in JPB

Journal of

Plant Biotechnology

pISSN 1229-2818
eISSN 2384-1397
qr-code Download