Research Article

J Plant Biotechnol 2015; 42(1): 34-42

Published online March 31, 2015

© The Korean Society of Plant Biotechnology

Production of biomass and bioactive compounds from adventitious root cultures of Polygonum multiflorum using air-lift bioreactors

Kyung-Ju Lee, Youngki Park, Ja-Young Kim, Taek-Kyu Jeong, Kyung-Seop Yun, Kee-Yoeup Paek, So-Young Park

Correspondence to :

S.-Y. Park

Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea

e-mail: soypark7@cbnu.ac.kr

Received: 9 March 2015; Revised: 23 March 2015; Accepted: 23 March 2015

Abstract

This study was conducted to investigate the productivity of biomass and antioxidant compounds in Polygonum multiflorum by culturing explants in air-lift bioreactor containing Murashige and Skoog (MS) medium, by adding different concentrations of auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], sucrose, methyl jasmonate (MeJA), and salicylic acid (SA). Results of this study revealed that the explants culturing on the medium supplemented with 9.84 μM IBA and 50 g/L sucrose were observed to have higher productivity of biomass and bioactive compound than other treatments used. Thus, we expect that these results will be helpful for large-scale production of biomass and antioxidant compounds from Polygonum multiflorum.

Keywords Bioreactor, Polygonum multiflorum, Bioactive compounds, Adventitious root, Biomass

Article

Research Article

J Plant Biotechnol 2015; 42(1): 34-42

Published online March 31, 2015

Copyright © The Korean Society of Plant Biotechnology.

Production of biomass and bioactive compounds from adventitious root cultures of Polygonum multiflorum using air-lift bioreactors

Kyung-Ju Lee, Youngki Park, Ja-Young Kim, Taek-Kyu Jeong, Kyung-Seop Yun, Kee-Yoeup Paek, So-Young Park

Correspondence to:

S.-Y. Park

Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea

e-mail: soypark7@cbnu.ac.kr

Received: 9 March 2015; Revised: 23 March 2015; Accepted: 23 March 2015

Abstract

This study was conducted to investigate the productivity of biomass and antioxidant compounds in Polygonum multiflorum by culturing explants in air-lift bioreactor containing Murashige and Skoog (MS) medium, by adding different concentrations of auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], sucrose, methyl jasmonate (MeJA), and salicylic acid (SA). Results of this study revealed that the explants culturing on the medium supplemented with 9.84 μM IBA and 50 g/L sucrose were observed to have higher productivity of biomass and bioactive compound than other treatments used. Thus, we expect that these results will be helpful for large-scale production of biomass and antioxidant compounds from Polygonum multiflorum.

Keywords: Bioreactor, Polygonum multiflorum, Bioactive compounds, Adventitious root, Biomass

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