Research Article

J Plant Biotechnol 2014; 41(3): 134-139

Published online September 30, 2014

© The Korean Society of Plant Biotechnology

장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립

한은희, 손용완, 김만배, 신용욱, 조영손, 이신우

Received: 6 August 2014; Revised: 26 August 2014; Accepted: 22 September 2014

Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings

Eun-Heui Han, Yong-Wan Son, Man-Bae Kim, Yong-Wook Shin, Young-Son Cho, Shin-Woo Lee

Correspondence to : Shin-Woo Lee

Received: 6 August 2014; Revised: 26 August 2014; Accepted: 22 September 2014

Abstract

The aim of this study was to establish the condition of regeneration for white balloon flower (Platycodon grandiflorum DC. cv. Jangback) and to manage for the raising of seedling with in vitro regenerated plants. It was examined that 0.5 mg/L of NAA and 1.0 mg/L of BA was the best composition for the callus and shoot induction (up to 600%). NAA was better than IBA for the induction of root and it took 16.9 days for the induction of rooting on the MS soild media containing 0.5 mg/L of NAA and the final rooting ratio was up to 75%. Out of 5 different bed soils purchased from local market, “Tosil” was identified to be the best for the acclimation and growth of in vitro regenerated balloon flower. In detail, on 8 weeks after planting of in vitro regenerated plants in pots containing “Tosil” bed soils, the plant hight was increased up to 2-fold (12.8 cm), 3.5-fold (27) for the number of leaf and 1.5-fold (4.5 cm) for the leaf length when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to prevent the occurrence of blue balloon flower in the massive cultivated area of white balloon flower by providing the seedlings raised from in vitro regenerated plants.

Keywords Balloon flower, Seedlings, Regeneration, Bed soils, Growth regulators, Mass propagation

Article

Research Article

J Plant Biotechnol 2014; 41(3): 134-139

Published online September 30, 2014

Copyright © The Korean Society of Plant Biotechnology.

장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립

한은희, 손용완, 김만배, 신용욱, 조영손, 이신우

Received: 6 August 2014; Revised: 26 August 2014; Accepted: 22 September 2014

Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings

Eun-Heui Han, Yong-Wan Son, Man-Bae Kim, Yong-Wook Shin, Young-Son Cho, Shin-Woo Lee

Correspondence to:Shin-Woo Lee

Received: 6 August 2014; Revised: 26 August 2014; Accepted: 22 September 2014

Abstract

The aim of this study was to establish the condition of regeneration for white balloon flower (Platycodon grandiflorum DC. cv. Jangback) and to manage for the raising of seedling with in vitro regenerated plants. It was examined that 0.5 mg/L of NAA and 1.0 mg/L of BA was the best composition for the callus and shoot induction (up to 600%). NAA was better than IBA for the induction of root and it took 16.9 days for the induction of rooting on the MS soild media containing 0.5 mg/L of NAA and the final rooting ratio was up to 75%. Out of 5 different bed soils purchased from local market, “Tosil” was identified to be the best for the acclimation and growth of in vitro regenerated balloon flower. In detail, on 8 weeks after planting of in vitro regenerated plants in pots containing “Tosil” bed soils, the plant hight was increased up to 2-fold (12.8 cm), 3.5-fold (27) for the number of leaf and 1.5-fold (4.5 cm) for the leaf length when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to prevent the occurrence of blue balloon flower in the massive cultivated area of white balloon flower by providing the seedlings raised from in vitro regenerated plants.

Keywords: Balloon flower, Seedlings, Regeneration, Bed soils, Growth regulators, Mass propagation

JPB
Vol 51. 2024

Stats or Metrics

Share this article on

  • line

Related articles in JPB

Journal of

Plant Biotechnology

pISSN 1229-2818
eISSN 2384-1397
qr-code Download