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J Plant Biotechnol (2023) 50:183-189

Published online October 19, 2023

https://doi.org/10.5010/JPB.2023.50.023.183

© The Korean Society of Plant Biotechnology

Influence of basal medium formulations and silver nanoparticles on in vitro plant growth in gerbera

Hyun Hee Kang・Aung Htay Naing・Junping Xu・Mi Young Chung・Su Young Lee・Jeung-Sul Han・ Chang Kil Kim

Department of Horticultural Science, Kyungpook National University, Daegu 41566, South Korea Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, China Department of Agricultural Education, Sunchon National University, Suncheon, South Korea Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, South Korea

Correspondence to : e-mail: ckkim@knu.ac.kr

Received: 8 September 2023; Revised: 17 September 2023; Accepted: 17 September 2023

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. ‘Shiny Pink’ and ‘Pink Holic’ exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, ‘Snow Dream,’ ‘Harmony,’ and ‘Breeze’ remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in ‘Breeze,’ ‘Harmony,’ and ‘Snow Dream’ than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in ‘Pink Holic,’ particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

Keywords Leaf yellowing, MS medium types, sliver nanoparticles, Plant growth parameters, SPAD values

Article

Research Article

J Plant Biotechnol 2023; 50(1): 183-189

Published online October 19, 2023 https://doi.org/10.5010/JPB.2023.50.023.183

Copyright © The Korean Society of Plant Biotechnology.

Influence of basal medium formulations and silver nanoparticles on in vitro plant growth in gerbera

Hyun Hee Kang・Aung Htay Naing・Junping Xu・Mi Young Chung・Su Young Lee・Jeung-Sul Han・ Chang Kil Kim

Department of Horticultural Science, Kyungpook National University, Daegu 41566, South Korea Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, China Department of Agricultural Education, Sunchon National University, Suncheon, South Korea Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, South Korea

Correspondence to:e-mail: ckkim@knu.ac.kr

Received: 8 September 2023; Revised: 17 September 2023; Accepted: 17 September 2023

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. ‘Shiny Pink’ and ‘Pink Holic’ exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, ‘Snow Dream,’ ‘Harmony,’ and ‘Breeze’ remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in ‘Breeze,’ ‘Harmony,’ and ‘Snow Dream’ than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in ‘Pink Holic,’ particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

Keywords: Leaf yellowing, MS medium types, sliver nanoparticles, Plant growth parameters, SPAD values

Fig 1.

Figure 1.Illustration of growth status and leaf color (yellowing or greening) of different gerbera cultivars cultured on two different types of MS media, each containing FeNaEDTA or FeEDDHA. These images were taken after four weeks of culture
Journal of Plant Biotechnology 2023; 50: 183-189https://doi.org/10.5010/JPB.2023.50.023.183

Fig 2.

Figure 2.Illustration of SPAD values and plant growth parameters (plant height, leaf length, leaf width, and petiole length) of different gerbera cultivars cultured on two different types of MS media, each containing FeNaEDTA or FeEDDHA. These data were taken after four weeks of culture. Data represent the mean values of three replicates, and error bars indicate the standard error. Means with asterisk(s) are statistically significant (T-test, **p < 0.01, *p < 0.05). ns= non-significant
Journal of Plant Biotechnology 2023; 50: 183-189https://doi.org/10.5010/JPB.2023.50.023.183

Fig 3.

Figure 3.Illustration of plant growth status, leaf color (yellowing or greening), and SPAD values of gerbera ‘Pink Holic’ cultured on two different types of MS media, each containing FeNaEDTA or FeEDDHA. These media were supplemented with varying concentrations of AgNPs (0, 1, 1.5, and 2 mg/L). These images were taken after four weeks of culture. Data represent the mean values of three replicates, and error bars indicate the standard error
Journal of Plant Biotechnology 2023; 50: 183-189https://doi.org/10.5010/JPB.2023.50.023.183

Fig 4.

Figure 4.Illustration of plant growth parameters (plant height, leaf length, leaf width, and petiole length) of gerbera ‘Pink Holic’ cultured on two different types of MS media, each containing FeNaEDTA or FeEDDHA. These media were supplemented with varying concentrations of AgNPs (0, 1, 1.5, and 2 mg/L). These data were taken after four weeks of culture. Data represent the mean values of three replicates, and error bars indicate the standard error
Journal of Plant Biotechnology 2023; 50: 183-189https://doi.org/10.5010/JPB.2023.50.023.183
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