Journal of

Plant Biotechnology

Agrobacterium-mediated genetic transformation of embryo axis explants of chickpea cv. JAKI9218 with the LBA4404 strain harboring pCAMBIA 2301 with nptII and gusA genes. (a) Shoot bud induction from explants in SIM supplemented with cefotaxime (250 mg/l) and kanamycin (25 mg/l) after 1 week, (b) multiple shoot bud induction from explants after 4 weeks in SIM supplemented with cefotaxime (250 mg/l) and kanamycin (100 mg/l), (c) transient GUS expression in putative transgenic shoot after 3 weeks, (d) putative transgenic plants after root formation on RIM supplemented with cefotaxime (100 mg/l) and kanamycin (50 mg/l), and (e) Acclimatization of putative transgenic plant in a pot under greenhouse conditions. GUS: β-glucuronidase; RIM: root induction medium; SIM: shoot induction medium|@|~(^,^)~|@|Molecular confirmation of chickpea cv. JAKI9218 transgenic plants and analysis for the presence of the gusA gene in putative transgenic plants in the T0 generation using gusA-specific primers. gus: ß-glucuronidase; lanes 1-6: putative transgenic plants in the T0 generation; M: marker DNA ladder (1 kb ladder, Thermo Fisher Scientific); NT: no template (negative control); P: positive control (plasmid DNA); WT: wild type (control plant DNA)

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